背景:美容医学中已经采用了各种技术来对抗皮肤老化,特别是面部区域。透明质酸用于提高水分水平和细胞外基质分子。这项研究旨在通过皮内显微注射从组织学上评估低分子量透明质酸片段与氨基酸(HAAM)结合对面部皮肤嫩肤的影响。
方法:共有20名妇女,平均年龄为45岁,年龄在35至64岁之间,参与研究,其中绝经期8人,育龄期12人。使用中速疗法对患者施用HAAM。治疗前和治疗后三个月,每位患者都接受了小的圆形穿孔活检.超声检查采用B型,捕获纵向或横向方向的2D图像,频率范围从5到13兆赫(MYLABX8,ESAOTE,Genova,意大利)。共进行了60次超声检查,治疗前30例,治疗后30例。
结果:组织学分析表明,成纤维细胞活性增加,导致III型网状胶原蛋白的产生,以及血管数量和表皮厚度的增加。然而,对治疗前后的超声资料进行分析,结果显示,治疗前后皮肤厚度无统计学差异,下巴和下颌角。
结论:组织学评估表明HAAM的皮下浸润对面部皮肤的真皮具有实质性影响。
BACKGROUND: Various techniques have been employed in aesthetic medicine to combat skin aging, in particular that of the facial region. Hyaluronic acid is utilized to enhance moisture levels and extracellular matrix molecules. This study aims to histologically assess the effects of low molecular weight hyaluronic acid fragments combined with amino acids (HAAM) on facial skin rejuvenation through intradermal microinjections.
METHODS: A total of twenty women, with an average age of 45 and ranging from 35 to 64 years old, participated in the study, including 8 in menopause and 12 in the childbearing age group. Mesotherapy was used to administer HAAM to the patients. Prior to and three months after the treatment, each patient underwent small circular punch biopsies. Ultrasound examinations were conducted using B-mode, capturing 2D images in longitudinal or transverse orientations with frequencies ranging from 5 to 13 Mega-hertz (MY LAB X8, ESAOTE, Genova, Italy). A total of 60 ultrasound examinations were taken, with 30 collected before treatment and 30 after treatment.
RESULTS: The histological analysis demonstrates an increase in
fibroblast activity resulting in the production of Type III reticular collagen, as well as an increased number of blood vessels and epidermal thickness. However, the analysis of ultrasound data before and after treatment showed no statistical difference in skin thickness in malar area, chin and mandibular angle.
CONCLUSIONS: Histological assessments indicate that subcutaneous infiltration of HAAM has a substantial impact on the dermis of facial skin.