从大曲中筛选出可以通过发酵酒糟释放阿魏酸(FA)的微生物聚生体FA12。Tabaiella,Comamonadaceae,通过16SrRNA基因测序,FA12中的苍白杆菌含量很高。在以酒糟为介质的长期驯化过程中,FA12的生物量保持稳定,发酵液的pH值也相对稳定。同时,纤维素酶的活性,木聚糖酶,FA12分泌的阿魏酸酯酶在0.2350-0.4470、0.1917-0.3078和0.103-0.1595U/mL范围内稳定,分别,FA的释放量可达133.77μg/g。证明该微生物聚生体具有良好的遗传稳定性。此外,利用扫描电镜(SEM)分析了发酵前后酒糟中木质纤维素的结构变化,X射线衍射(XRD)和傅里叶变换红外光谱(FTIR),并检测了发酵前后酒糟重和木质纤维素含量的变化。这些结果都证实了FA12具有降解酒糟的功能。在这项研究中,我们探索了一种利用微生物群落从酒糟中释放FA并降解废物中木质纤维素的方法,这为高价值的损失废弃物的应用开辟了新的途径。
The microbial consortium FA12 that can release ferulic acid (FA) by fermenting distiller\'s grains was screened from Daqu. Taibaiella, Comamonadaceae, and Ochrobacum were highly abundant in FA12 by 16S rRNA gene sequencing. In the process of long-term acclimation with distiller\'s grains as a medium, the biomass of FA12 remained stable, and the pH value of fermentation liquid was also relatively stable. Meanwhile, the activities of cellulase, xylanase, and feruloyl esterase secreted by FA12 were stable in the ranges of 0.2350-0.4470, 0.1917-0.3078, and 0.1103-0.1595 U/mL, respectively, and the release of FA could reach 133.77 μg/g. It is proven that the microbial consortium has good genetic stability. In addition, the structural changes of lignocellulose in distiller\'s grains before and after fermentation were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR), and the changes of distiller\'s grains weight and lignocellulose content before and after fermentation were also detected. These results all confirmed that FA12 had the function of degrading distiller\'s grains. In this study, we explored a method to use microbial communities to release FA from distiller\'s grains and degrade lignocellulose in the waste, which opened up a new way for the application of the high value of lost waste.