生物标志物是特定的分子,组织学,或正常或致病性生物过程的生理特征,并且在胃肠道线虫(GINs)的诊断中很有前途。尽管一些生物标志物已被Ostertagiasp。在温带地区饲养的牛中,热带地区缺乏信息。该项目的目的是评估潜在的生物标志物并验证最有希望的生物标志物。在第一项研究中,自然感染GINs的36头牛(Nelore品种)分为两组:感染(未用驱虫药治疗)和治疗(在第0、7、14、21、28、42和56天用芬苯达唑治疗)。感兴趣的变量是活体重,粪便卵数,血象,血清生化标志物,磷,胃泌素,和胃蛋白酶原。第二步,在Nelore品种的牛中评估了胃蛋白酶原,分布在三组中:感染(未用驱虫药治疗),MOX(用莫西丁治疗),和IVM+BZD(用伊维菌素+阿苯达唑处理)。在第一项研究中,两组之间的体重没有差异,白蛋白,血细胞比容(红细胞体积[CV]),红细胞,或血红蛋白。发现胃蛋白酶原与CV和白蛋白之间呈负相关,白蛋白与Haemonchussp的百分比呈负相关。在粪便培养中。在生物标志物中,只有胃蛋白酶原分化治疗和感染(从研究的第28天开始)。在第二项研究中,驱虫治疗后发现胃蛋白酶原减少。因此,胃蛋白酶原是热带地区自然感染Haemonchus和Cooperia的牛中蠕虫的有希望的生物标志物。
Biomarkers are specific molecular, histological, or physiological characteristics of normal or pathogenic biological processes and are promising in the diagnosis of gastrointestinal nematodes (GINs). Although some biomarkers have been validated for infection by Ostertagia sp. in cattle raised in temperate regions, there is a lack of information for tropical regions. The aim of this project was to assess potential biomarkers and validate the most promising. In the first study, 36 bovines (Nelore breed) naturally infected by GINs were distributed into two groups: infected (not treated with anthelmintic) and treated (treated with
fenbendazole on days 0, 7, 14, 21, 28, 42, and 56). The variables of interest were live weight, fecal egg count, hemogram, serum biochemical markers, phosphorus, gastrin, and pepsinogen. In the second step, pepsinogen was assessed in cattle of the Nelore breed distributed among three groups: infected (not treated with anthelmintic), MOX (treated with moxidectin), and IVM + BZD (treated with ivermectin + albendazole). In the first study, no difference between groups was found for weight, albumin, hematocrit (corpuscular volume [CV]), erythrocytes, or hemoglobin. Negative correlations were found between pepsinogen and both CV and albumin, and albumin was negatively correlated with the percentage of Haemonchus sp. in the fecal culture. Among the biomarkers, only pepsinogen differentiated treated and infected (beginning with the 28th day of the study). In the second study, a reduction in pepsinogen was found after anthelmintic treatment. Therefore, pepsinogen is a promising biomarker of worms in cattle naturally infected by the genera Haemonchus and Cooperia in tropical areas.