未经授权:桥本甲状腺炎,自身免疫性甲状腺疾病,在世界范围内显示出很高的发病率,尤其是女性。桥本甲状腺炎患者在桥本甲状腺炎的发生和进展过程中甲状腺功能减退的风险增加。近年来,代谢组学已广泛应用于自身免疫性疾病,尤其是甲状腺疾病.然而,桥本甲状腺炎的代谢物分析仍然缺失。
未经评估:共收集了92个样本,对照组35例,桥本甲状腺炎合并甲状腺功能正常组30例,桥本甲状腺炎合并亚临床甲状腺功能减退症组27例。SPSS25.0用于统计分析和ROC曲线,SIMCA14.0,多因素分析的元分析,和原点2021进行相关性分析。
未经鉴定:鉴定了21种代谢物。从对照组和HTE组获得10种代谢物,对照组与HTS组之间有8种血清代谢产物异常,与HTS相比,3种代谢物来自HTE组。京都百科全书的基因和基因组富集分析表明,脂肪酸降解,精氨酸,脯氨酸代谢对HTE有显著影响,而赖氨酸降解,酪氨酸代谢在HTS组,与对照组相比。在HTE和HTS组之间的比较中,缬氨酸,亮氨酸,异亮氨酸降解和缬氨酸,亮氨酸,和异亮氨酸的生物合成存在关键作用。相关性分析显示临床均与代谢物无关。ROC曲线表示SM,LPC,PC可以有效地从健康个体中识别不同临床阶段的HT患者。
未经证实:血清代谢产物在HT中发生改变。磷脂,如SM,LPC,PC影响桥本甲状腺炎的发病机制。脂肪酸降解和赖氨酸降解途径对HT的不同临床阶段有影响。
Hashimoto\'s thyroiditis, an autoimmune thyroid disease, shows high morbidity worldwide, particularly in female. Patients with Hashimoto\'s thyroiditis have an increasing risk of hypothyroidism during the occurrence and progression of Hashimoto\'s thyroiditis. In recent years, metabolomics has been widely applied in autoimmune diseases, especially thyroid disorders. However, metabolites analysis in Hashimoto\'s thyroiditis is still absent.
A total of 92 samples were collected, including 35 cases in the control group, 30 cases in the Hashimoto\'s thyroiditis with
euthyroidism group, and 27 cases in the Hashimoto\'s thyroiditis with subclinical hypothyroidism group. SPSS 25.0 for statistical analysis and ROC curve, SIMCA 14.0, Metaboanalysis for multifactor analysis, and Origin 2021 for correlation analysis.
21 metabolites were identified. 10 metabolites were obtained from control group versus HTE group, 8 serum metabolites were abnormal between control group and HTS group, 3 metabolites were derived from HTE group versus HTS. Kyoto Encyclopedia of Genes and Genomes Enrichment analysis showed that fatty acid degradation, Arginine, and proline metabolism have a significant impact on HTE, while lysine degradation, tyrosine metabolism play an important role HTS group, compared to control group. In the comparison between the HTE and HTS group, Valine, leucine, and isoleucine degradation and Valine, leucine, and isoleucine biosynthesis exists a key role. Correlation analysis shows clinical are not related to metabolites. ROC curve indicates SM, LPC, PC can efficiency in identification patients with HT in different clinical stage from healthy individuals.
Serum metabolites were changed in HT. Phospholipids such as SM, LPC, PC influence the pathogenesis of Hashimoto\'s thyroiditis. Fatty acid degradation and lysine degradation pathways have an impact on different clinical stage of HT.