酶处理木质纤维素生物质已成为制备纳米纤维素(NC)的趋势,但必须优化工艺以保证高产量和结晶度。这项研究提供了对使用栽培真菌纤维素酶和木聚糖酶的创新方案的见解,以使用五因素四水平的Taguchi正交设计来优化参数,以改善粗油棕榈叶(OPL)的NC生产。即底物和酶负载,表面活性剂浓度,孵育温度和时间。统计结果揭示了生产NC的最佳条件(66.06%结晶度,43.59%的产率)需要10%(w/v)的底物,1%(v/v)酶,1.4%(w/v)Tween-80,在30°C孵育72小时同样,使用2.5%(w/v)底物获得最高的糖产率(47.07%),2.0%(v/v)酶,2.0%(w/v)Tween-80,60°C孵育72小时本研究中使用的辅助酶,即,木聚糖酶,产生更高的结晶度NC,显示8和12nm之间的宽度和>1μm的长度和47.07%产率的糖。因此,我们的发现证明,优化原始OPL的单步酶水解可以令人满意地产生相对结晶的NC和糖产量,以进一步转化为生物纳米复合材料和生物燃料。这项研究提出了一个简单的,数控合成的创新方案显示出与传统制备的数控相当的特性,这对材料的商业化至关重要。
Enzymatic treatment on lignocellulosic biomass has become a trend in preparing nanocellulose (NC), but the process must be optimized to guarantee high production yield and crystallinity. This study offers insights into an innovative protocol using cultivated fungal cellulase and xylanase to improve NC production from raw oil palm leaves (OPL) using five-factor-four-level Taguchi orthogonal design for optimizing parameters, namely substrate and enzyme loading, surfactant concentration, incubation temperature and time. Statistical results revealed the best condition for producing NC (66.06 % crystallinity, 43.59 % yield) required 10 % (w/v) substrate, 1 % (v/v) enzyme, 1.4 % (w/v) Tween-80, with 72-h incubation at 30 °C. Likewise, the highest sugar yield (47.07 %) was obtained using 2.5 % (w/v) substrate, 2.0 % (v/v) enzyme, 2.0 % (w/v) Tween-80, with 72-h incubation at 60 °C. The auxiliary enzymes used in this study, i.e., xylanase, produced higher crystallinity NC, showing widths between 8 and 12 nm and lengths >1 μm and sugars at 47.07 % yield. Thus, our findings proved that optimizing the single-step enzymatic hydrolysis of raw OPL could satisfactorily produce relatively crystalline NC and sugar yield for further transformation into bio-nanocomposites and biofuels. This study presented a simple, innovative protocol for NC synthesis showing characteristics comparable to the traditionally-prepared NC, which is vital for material\'s commercialization.