背景:人们经常接触甲醛,一种易挥发的有毒气体,在室内环境中。特别是,解剖学家,病理学家,组织学专家,那些参与防腐的人由于他们的工作而不断暴露于更高的甲醛含量。本研究旨在探讨N-乙酰半胱氨酸对甲醛暴露雄性大鼠内皮抑素和humanin值的影响。
方法:在研究中,28只12-14周龄的雄性Spraque-Dawley大鼠(每组7只:对照组,甲醛组,N-乙酰半胱氨酸基团,使用甲醛+N-乙酰半胱氨酸组)。四周后,动物被斩首处死。斩首之后,通过酶联免疫分析(ELISA)方法研究了大鼠血清中内皮抑素和humanin的水平。在所有分析中,p<0.05被认为是统计学上显著的。
结果:在大鼠血清中检查Humanin和内皮抑素值。当组间比较humanin水平时,甲醛组与对照组(p<0.05)和N-乙酰半胱氨酸组(p<0.05)之间存在统计学上的显着差异。在甲醛+N-乙酰半胱氨酸基团中,确定人素水平由于甲醛暴露而受损,用N-乙酰半胱氨酸接近对照组值。当比较各组的内皮抑素水平时,仅在甲醛组和N-乙酰半胱氨酸组之间发现统计学意义(p<0.05)。在甲醛+N-乙酰半胱氨酸基团中,确定内皮抑素水平由于甲醛暴露而受损,用N-乙酰半胱氨酸接近对照组值。
结论:在这项研究中,首次证明了N-乙酰半胱氨酸对甲醛暴露大鼠humanin和内皮抑素的影响。甲醛暴露对大鼠血清中的人肽和内皮抑素水平产生负面影响。N-乙酰半胱氨酸改善了甲醛的负面影响,使humanin和内皮抑素水平更接近健康对照组。
BACKGROUND: People are constantly exposed to formaldehyde, a volatile and poisonous gas, in indoor environments. In particular, anatomists, pathologists, histologists, and those involved in embalming are exposed to higher amounts of formaldehyde continuously due to their work. This study aimed to investigate the effect of N-acetylcysteine on
endostatin and humanin values in male rats exposed to experimental formaldehyde.
METHODS: In the study, 28 male Spraque-Dawley rats aged 12-14 weeks (seven animals in each group: control group, formaldehyde group, N-acetylcysteine group, formaldehyde+N-acetylcysteine group) were used. Four weeks later, the animals were sacrificed by decapitation. Following decapitation, endostatin and humanin levels in the serum of rats were studied by the enzyme-linked immunoassay (ELISA) method. In all analyses, p<0.05 was accepted as statistically significant.
RESULTS: Humanin and
endostatin values were checked in the serum of rats. When humanin levels were compared between groups, a statistically significant difference was found between the formaldehyde group and both the control group (p<0.05) and the N-acetylcysteine group (p<0.05). In the formaldehyde+N-acetylcysteine group, it was determined that the humanin level was impaired due to formaldehyde exposure, approaching the control group values with the administered N-acetylcysteine. When the
endostatin level was compared between the groups, a statistical significance (p<0.05) was found only between the formaldehyde group and the N-acetylcysteine group. In the formaldehyde+N-acetylcysteine group, it was determined that the
endostatin level was impaired due to formaldehyde exposure, approaching the control group values with the administered N-acetylcysteine.
CONCLUSIONS: In this study, the effects of N-acetylcysteine on humanin and endostatin on rats exposed to formaldehyde were demonstrated for the first time. Formaldehyde exposure negatively affected humanin and
endostatin levels in rat sera. N-acetylcysteine ameliorated the negative effects of formaldehyde, bringing humanin and endostatin levels closer to the healthy control group.