Phellinuscaribaeo-quercicola是一种担子菌,在这项研究中,从健康且无症状的InularacemosaHook的叶子中分离为内生菌。f.,一种生长在克什米尔喜马拉雅的重要药草。这项研究结合了形态学,分子和系统发育技术来鉴定内生真菌,使用nrDNA的ITS序列。进行了分离叶测定以评估真菌内生菌的致病性,表明其与宿主的相互共生关系。作者还研究了分离的内生菌株的抗真菌潜力,以确定其作为生物防治剂的用途。研究表明,卡利贝奥-槲寄生INL3-2菌株对四种关键的真菌植物病原体表现出生物防治活性,这些真菌植物病原体会导致重大的农艺和经济损失:黄曲霉,黑曲霉,镰刀菌,和尖孢镰刀菌.值得注意的是,P.caribaeo-quercicolaINL3-2菌株对黄曲霉非常有效,抑制百分比为57.63%。此外,本研究以乙酸乙酯和甲醇为溶剂,研究了卡里贝奥-quercicolaINL3-2菌株粗提物的抗氧化活性。结果表明,卡里博奥-槲树的甲醇部分具有作为抗氧化剂的潜力,IC50值为171.90±1.15µg/mL。这项调查是首次,标志着这种真菌担子菌的初步报告,P.Caribaeo-quercicola,作为与药用植物相关的内生菌。这项研究的发现突出了P.caribaeo-quercicolaINL3-2菌株作为双重作用剂的潜力,具有生物控制和抗氧化特性,与菊花的药用特性一致。这种内生真菌可能是用于农业的天然化合物的有希望的来源,医学,和超越。
Phellinus caribaeo-quercicola is a basidiomycetous fungus, isolated as an
endophyte in this study from the healthy and symptomless leaves of Inula racemosa Hook. f., an important medicinal herb growing in Kashmir Himalaya. This study combines morphological, molecular and phylogenetic techniques to identify the fungal
endophyte, using the ITS sequence of nrDNA. A detached leaf assay was conducted to assess the pathogenicity of the fungal
endophyte suggesting its mutually symbiotic relationship with the host. The authors also investigated the antifungal potential of the isolated endophytic strain to ascertain its use as a biocontrol agent. The study shows that P. caribaeo-quercicola INL3-2 strain exhibits biocontrol activity against four key fungal phytopathogens that cause significant agronomic and economic losses: Aspergillus flavus, Aspergillus niger, Fusarium solani, and Fusarium oxysporum. Notably, P. caribaeo-quercicola INL3-2 strain is highly effective against A. flavus, with an inhibition percentage of 57.63%. In addition, this study investigates the antioxidant activity of P. caribaeo-quercicola INL3-2 strain crude extracts using ethyl acetate and methanol as solvents. The results showed that the methanolic fraction of P. caribaeo-quercicola exhibits potential as an antioxidant agent, with an IC50 value of 171.90 ± 1.15 µg/mL. This investigation is first of its kind and marks the initial report of this fungal basidiomycete, P. caribaeo-quercicola, as an
endophyte associated with a medicinal plant. The findings of this study highlight the potential of P. caribaeo-quercicola INL3-2 strain as a dual-action agent with both biocontrol and antioxidant properties consistent with the medicinal properties of Inula racemosa. This endophytic fungus could be a promising source of natural compounds for use in agriculture, medicine, and beyond.