3-甲基组氨酸(3-MeHis)越来越多地用作肌肉蛋白质分解的指示剂。一个敏感的发展,简单,3-MeHis测定的非侵入性方法在临床实践中很重要。在这里,一个敏感的,简单,提出了使用铱(III)溶剂络合物([Ir(dfppy)2(DMSO)Cl]定量尿液中3-MeHis的非侵入性电化学发光(ECL)方法,dfppy=2-(2,4-二氟苯基)吡啶,Ir-DMSO)作为信号试剂。研究了Ir-DMSO对3-MeHis的光致发光(PL)和ECL响应。由于Ir-DMSO和3-MeHis的咪唑基团之间的配位识别,在3-MeHis存在下Ir-DMSO的ECL强度增强。基于ECL强度的增强,可以在5至25μM的范围内灵敏地检测3-MeHis。检测极限为0.4μM。这是用于3-MeHis定量的ECL方法的第一份报告。Further,为了研究基于Ir-DMSO的ECL方法在实际应用中的可行性,将开发的ECL方法应用于28名健康志愿者和2名患者的尿液样本中的3-MeHis测定。来自肥胖和肾脏疾病住院患者和健康个体的尿液样品通过Ir-DMSO的ECL反应进行区分。所提出的基于铱(III)溶剂络合物与3-MeHis的咪唑基团之间的配位识别的ECL方法允许廉价,快,非侵入性,以及灵敏检测尿液中的3-MeHis,这对于在临床实践中评估大量患者进行常规分析是有希望的。
3-Methylhistidine (3-MeHis) is increasingly used as an indicator of muscle protein breakdown. The development of a sensitive, simple, and non-invasive method for 3-MeHis assay is important in clinical practice. Herein, a sensitive, simple, and non-invasive electrogenerated chemiluminescence (ECL) method was proposed for the quantitation of 3-MeHis in urine by using an iridium(III) solvent complex ([Ir(dfppy)2(DMSO)Cl], dfppy = 2-(2,4-difluorophenyl)pyridine, Ir-DMSO) as a signal reagent. The photoluminescence (PL) and ECL responses of Ir-DMSO to 3-MeHis were studied. The ECL intensity of Ir-DMSO was enhanced in the presence of 3-MeHis because of the coordination recognition between Ir-DMSO and the imidazole group of 3-MeHis. Based on the enhancement of ECL intensity, 3-MeHis can be sensitively detected in the range of 5 to 25 μM. The detection limit was 0.4 μM. This is the first report of an ECL method for the quantitation of 3-MeHis. Further, to investigate the feasibility of the Ir-DMSO-based ECL method in practical applications, the developed ECL method was applied for 3-MeHis assay in urine samples of 28 healthy volunteers and 2 patients. The urine samples from patients hospitalized with obesity and kidney disease and healthy individuals were distinguished by the ECL responses of Ir-DMSO. The proposed ECL method based on the coordination recognition between iridium(III) solvent complex and the imidazole group of 3-MeHis allows inexpensive, fast, non-invasive, and sensitive detection of 3-MeHis in urine, which is promising for assessing large volumes of patients for routine analysis in clinical practices.