Abstract：
Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into an optical microscopy. The orthogonality of the electrochemical trigger and the optical readout distinguishes it from classic microscopy and electrochemical techniques, owing to its near-zero background, remarkable sensitivity, and absence of photobleaching and phototoxicity. In this minireview, we summarize the recent advances in ECL imaging technology, emphasizing original configurations which enable the imaging of biological entities and the improvement of the analytical properties by increasing the complexity and multiplexing of bioassays. Additionally, mapping the (electro)chemical reactivity in space provides valuable information on nanomaterials and facilitates deciphering ECL mechanisms for improving their performances in diagnostics and (electro)catalysis. Finally, we highlight the recent achievements in imaging at the ultimate limits of single molecules, single photons or single chemical reactions, and the current challenges to translate the ECL imaging advances to other fields such as material science, catalysis and biology.
摘要：
电化学发光（ECL）正迅速从分析方法发展成为光学显微镜。电化学触发器和光学读出的正交性将其与经典的显微镜和电化学技术区分开来，由于其接近零的背景，非凡的灵敏度,没有光漂白和光毒性。在这次审查中,我们总结了ECL成像技术的最新进展，通过增加生物测定的复杂性和多路复用，强调能够对生物实体进行成像和改善分析特性的原始配置。此外,绘制空间中的（电）化学反应性图提供了有关纳米材料的宝贵信息，并有助于破译ECL机制，以改善其在诊断和（电）催化中的性能。最后,我们强调了最近在单分子极限成像方面的成就，单光子或单一化学反应，以及当前将ECL成像进展转化为材料科学等其他领域的挑战，催化和生物学。
