节肢动物通过蚊子和蜱叮咬将虫媒病毒传播给人类和其他动物。黄病毒属,导致疾病,后遗症,和成千上万的死亡,主要在发展中国家和不发达国家,是公共卫生关注的虫媒病毒之一。鉴于早期和准确诊断的重要性,本文分析了直接检测黄病毒的方法,如逆转录环介导的等温扩增,微流体,局域表面等离子体共振,和表面增强拉曼散射,并展示了优势,缺点,以及在文献中报道的每种方法的研究中确定的检测限。在可用的不同方法中,必须平衡四个基本指标来确定理想的测试:良好的灵敏度,高特异性,低假阳性率,和快速的结果。在分析的方法中,逆转录环介导的等温扩增脱颖而出,由于结果在几分钟内可用,具有良好的敏感性和特异性;此外,这是最好的方法。
Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology.