Thrips传播的番茄斑点枯萎病原孢子病毒(TSWV)会导致花生(ArachishypogaeaL.)中的斑点枯萎病,并限制产量。育种计划一直在开发抗TSWV的品种,但是在栽培种质中对TSWV的抗性来源的可用性极为有限。二倍体野生花生物种可以作为重要的抗性来源,尽管存在倍性障碍(栽培花生是四倍体),由于诱导的种间异源四倍体杂种的知识和发展,它们在育种程序中的使用现在是可能的。这项研究通过在温室中通过蓟马介导的TSWV传播试验和蓟马摄食试验筛选了10种野生二倍体花生和6种诱导的异源四倍体基因型。评估了三个参数:TSWV感染百分比,病毒积累,和蓟马摄食损伤的时间严重程度。结果表明,在评估的基因型中,二倍体A.stenosperma登录名V10309及其衍生物诱导的同种四倍体ValSten1的TSWV感染率最低。同源四倍体BatDur1在蓟马释放后的每个星期对蓟马造成的损害最低,虽然二倍体A.batizocoi登录名K9484和A.duranensis登录名V14167在蓟马释放后一周减少了摄食损害,和二倍体A.valida登录名GK30011和A.batizocoi在蓟马释放后三周比其他动物减少了摄食损害。总的来说,二倍体物种及其异源四倍体杂种中的合理TSWV抗性的特征是TSWV感染百分比降低,病毒积累,和喂养的严重性。此外,一些二倍体和四倍体杂种对蓟马具有抗菌作用。这些结果证明了野生二倍体花生属物种和花生相容性诱导的异源四倍体对TSWV和蓟马的抗性。
Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) causes spotted wilt disease in peanut (Arachis hypogaea L.) and limits yield. Breeding programs have been developing TSWV-resistant cultivars, but availability of sources of resistance against TSWV in cultivated germplasm is extremely limited.
Diploid wild Arachis species can serve as important sources of resistance, and despite ploidy barriers (cultivated peanut is tetraploid), their usage in breeding programs is now possible because of the knowledge and development of induced interspecific allotetraploid hybrids. This study screened 10 wild
diploid Arachis and six induced allotetraploid genotypes via thrips-mediated TSWV transmission assays and thrips\' feeding assays in the greenhouse. Three parameters were evaluated: percent TSWV infection, virus accumulation, and temporal severity of thrips feeding injury. Results indicated that the
diploid A. stenosperma accession V10309 and its derivative-induced allotetraploid ValSten1 had the lowest TSWV infection incidences among the evaluated genotypes. Allotetraploid BatDur1 had the lowest thrips-inflicted damage at each week post thrips release, while diploid A. batizocoi accession K9484 and A. duranensis accession V14167 had reduced feeding damage one week post thrips release, and diploids A. valida accession GK30011 and A. batizocoi had reduced feeding damage three weeks post thrips releasethan the others. Overall, plausible TSWV resistance in
diploid species and their allotetraploid hybrids was characterized by reduced percent TSWV infection, virus accumulation, and feeding severity. Furthermore, a few diploids and tetraploid hybrids displayed antibiosis against thrips. These results document evidence for resistance against TSWV and thrips in wild
diploid Arachis species and peanut-compatible-induced allotetraploids.