Abstract：
Exposure to temperatures outside of a fish\'s optimal range results in suppression of the immune system, ultimately leaving aquaculture stocks susceptible to disease outbreaks. This effect is exacerbated in triploid fishes, which demonstrate greater susceptibility to stress than their diploid counterparts. This study investigates the impacts of acute heat stress on the abundance of immune transcripts and proteins in diploid and triploid Chinook salmon (Oncorhynchus tshawytscha), an important finfish crop. This study also demonstrates that acute heat stress induces significant increases in the abundance hsp70, hsp90 and il1b transcripts in the head kidneys, gills and heart ventricles of both diploid and triploid Chinook salmon. Widespread dysregulation of antigen-presentation transcripts was also observed in fish of both ploidies. These results suggest that acute heat stress activates acute-phase responses in Chinook salmon and dysregulates antigen presentation, potentially leaving fish more susceptible to infection. At the protein level, IL-1β was differentially expressed in the head kidney and ventricles of diploid and triploid salmon following heat shock. Differential expression of two tapasin-like proteins in diploid and triploid salmon subjected to heat shock was also observed. Altogether, these data indicate that diploid and triploid Chinook salmon respond differently to acute thermal stressors.
摘要：
暴露在鱼的最佳范围之外的温度会抑制免疫系统，最终使水产养殖种群容易受到疾病爆发的影响。这种效应在三倍体鱼类中加剧，比二倍体对应物更容易受到压力。这项研究调查了急性热应激对二倍体和三倍体奇努克鲑鱼（Oncorhynchustshawytscha）中免疫转录本和蛋白质丰度的影响，一种重要的有鳍鱼类作物。这项研究还表明，急性热应激诱导头肾中hsp70，hsp90和il1b转录物的丰度显着增加，二倍体和三倍体奇努克鲑鱼的ill和心室。在两种倍性鱼类中也观察到抗原呈递转录物的广泛失调。这些结果表明，急性热应激激活奇努克鲑鱼的急性期反应，并失调抗原呈递，可能使鱼类更容易受到感染。在蛋白质水平，热休克后，IL-1β在二倍体和三倍体鲑鱼的头肾和心室中差异表达。还观察到在遭受热休克的二倍体和三倍体鲑鱼中两种tapasin样蛋白的差异表达。总之,这些数据表明，二倍体和三倍体奇努克鲑鱼对急性热应激源的反应不同。
