SREBPs,SREBP1和SREBP2是调节脂质代谢的关键转录因子。SREBPs的加工涉及许多基因,比如scap,s1p,s2p,Cideb.这里,我们破译了scap的全长cDNA序列,srebp1,srebp2,s1p,s2p,来自黄色cat鱼Pelteobagrusfulvidraco的cideb和cidec。它们的全长cDNA序列范围为1587-3884bp,它们的ORF长度为1191-2979bp,编码396-992个氨基酸。预测了一些保守域,包括SCAP中的多个跨膜结构域,SREBP1和SREBP2中的bHLH-ZIP域,ApoB结合区,CIDEb中的ER靶向区和LD靶向区,CIDEc中的LD靶向区域,S1P中保守的催化位点和加工位点,和S2P中的跨膜螺旋结构域。可以在心脏中观察到它们的mRNA表达,脾,脾肝脏,肾,大脑,肌肉,肠和脂肪,但随着组织的变化。在大脑中还研究了其对高脂肪(HFD)和胆汁酸(BA)饮食的反应中mRNA表达的变化,心,肠,肾和脾组织。在大脑中,HFD显著增加了7个基因的mRNA表达(scap,srebp1,srebp2,s1p,s2p,cideb和cidec),BA减弱了scap的增加,srebp1,srebp2,s1p,s2p,HFD诱导的Cideb和CidecmRNA表达。在心中,HFD显着增加了六个基因的mRNA丰度(srebp1,srebp2,scap,s2p,cideb和cidec),和BA减弱了由HFD诱导的其mRNA丰度的增加。在肠道里,HFD增加了cideb,s1p和s2pmRNA丰度,和BA减弱了HFD诱导的mRNA丰度增加。在肾脏,HFD显着增加了scap,cidecc和s1pmRNA表达,和BA饮食减弱了其mRNA表达的增加。在脾脏里,HFD治疗增加了scap,srebp2,s1p和s2pmRNA表达,和BA饮食减弱HFD诱导的mRNA表达增加。一起来看,我们的研究阐明了特征,七个脂质代谢基因的表达谱和转录反应,这将为进一步探索其在鱼类中的功能和调控机制奠定良好的基础。
SREBPs, such as SREBP1 and SREBP2, were the key transcriptional factors regulating lipid metabolism. The processing of SREBPs involved many genes, such as scap, s1p, s2p, cideb. Here, we deciphered the full-length cDNA sequences of scap, srebp1, srebp2, s1p, s2p, cideb and cidec from yellow catfish Pelteobagrus fulvidraco. Their full-length cDNA sequences ranged from 1587 to 3884 bp, and their ORF length from 1191 to 2979 bp, encoding 396-992 amino acids. Some conservative domains were predicted, including the multiple transmembrane domains in SCAP, the bHLH-ZIP domain in SREBP1 and SREBP2, the ApoB binding region, ER targeting region and LD targeting region in CIDEb, the LD targeting region in the CIDEc, the conserved catalytic site and processing site in S1P, and the transmembrane helix domain in S2P. Their mRNA expression could be observed in the heart, spleen, liver, kidney, brain, muscle, intestine and adipose, but varied with tissues. The changes of their mRNA expression in responses to high-fat (HFD) and bile acid (BA) diets were also investigated in the brain, heart, intestine, kidney and spleen tissues. In the brain, HFD significantly increased the mRNA expression of seven genes (scap, srebp1, srebp2, s1p, s2p, cideb and cidec), and the BA attenuated the increase of scap, srebp1, srebp2, s1p, s2p, cideb and cidec mRNA expression induced by HFD. In the heart, HFD significantly increased the mRNA abundances of six genes (srebp1, srebp2, scap, s2p, cideb and cidec), and BA attenuated the increase of their mRNA abundances induced by HFD. In the intestine, HFD increased the cideb, s1p and s2p mRNA abundances, and BA attenuated the HFD-induced increment of their mRNA abundances. In the kidney, HFD significantly increased the scap, cidec and s1p mRNA expression, and BA diet attenuated the increment of their mRNA expression. In the spleen, HFD treatment increased the scap, srebp2, s1p and s2p mRNA expression, and BA diet attenuated HFD-induced increment of their mRNA expression. Taken together, our study elucidated the characterization, expression profiles and transcriptional response of seven lipid metabolic genes, which would serve as the good basis for the further exploration into their function and regulatory mechanism in fish.