背景:不愈合的糖尿病伤口是一种严重的并发症,与极度昏昏欲睡的伤口闭合和感染的高风险相关,导致截肢或肢体丧失,以及大量的医疗保健费用和患者的生活质量差。在诱发糖尿病的大鼠模型中,评估了蛋壳膜(ESM)和绿海藻(Ulvalactuca)提取物单独或组合的体内皮肤伤口愈合的作用。
方法:废母鸡ESM的微粉化粉末,榆树,和他们的1:1混合物是使用常规程序制备的。机械的,电气,和粉末的表面形态特征进行了检查,使用直接压缩,LCR阻抗,和扫描电子显微镜。ESM的影响,榆树,与标准Dermazin治疗相比,它们的混合物在诱发糖尿病的雄性Wistar白化病大鼠的伤口上进行了评估。使用ANOVA在所有大鼠组中进行基线和治疗3、7、14和21天的定量伤口愈合率。表皮再上皮化的定性组织学分析,角质形成细胞,基底膜,浸润淋巴细胞,胶原纤维,第21天的血管使用ImageJ处理程序进行。
结果:片剂的压缩强度测量显示,ESM和ESM+Ulvalactuca混合物的杨氏模量为44.14和27.17MPa,分别。此外,两个样品在1MHz时表现出相对较低的相对介电常数值,分别为6.62和6.95,分别,由于扫描电子显微镜显示ESM的多孔表面形态。在第21天,用ESM治疗的大鼠完全糖尿病伤口闭合,毛发再生,和99.49%的治愈率,与Dermazin的96.79%相比,Ulvalactuca的87.05%,混合物的90.23%,阴性对照仅为36.44%。形成良好的基底膜,分化良好的上皮细胞,在用ESM治疗的大鼠中,表皮细胞表面有规则的厚角质形成细胞伴随着伤口愈合,明显优于对照大鼠。
结论:磨碎的母鸡ESM粉末,一种低成本有效的生物材料,在预防组织损伤和促进糖尿病伤口愈合方面优于Ulvalactuca或其混合物,除了各种生物医学应用。
BACKGROUND: Nonhealing diabetic wounds are a serious complication associated with extremely lethargic wound closure and a high risk of infection, leading to amputation or limb loss, as well as substantial health care costs and a poor quality of life for the patient. The effects of either eggshell membrane (ESM) and green seaweed (Ulva lactuca) extracts alone or in combination were evaluated for in vivo skin wound healing in a rat model of induced diabetes.
METHODS: Micronized powders of waste hen ESM, Ulva lactuca, and their 1:1 mixture were prepared using regular procedures. The mechanical, electrical, and surface morphology characteristics of powders were examined using direct compression, LCR-impedancemetry, and scanning electron microscopy. The effect of ESM, Ulva lactuca, and their mixture as compared to standard Dermazin treatments were evaluated on wounds inflicted on male Wistar Albino rats with induced diabetes. Quantitative wound healing rates at baseline and at 3, 7, 14, and 21 days of treatments among all rat groups were conducted using ANOVA. Qualitative histological analysis of epidermal re-epithelization, keratinocytes, basement membrane, infiltrating lymphocytes, collagen fibrines, and blood vessels at day 21 were performed using Image J processing program.
RESULTS: Compressive strength measurements of tablets showed a Young\'s modulus of 44.14 and 27.17 MPa for the ESM and ESM + Ulva lactuca mixture, respectively. Moreover, both samples exhibited relatively low relative permittivity values of 6.62 and 6.95 at 1 MHz, respectively, due to the porous surface morphology of ESM shown by scanning electron microscopy. On day 21, rats treated with ESM had a complete diabetic wound closure, hair regrowth, and a healing rate of 99.49%, compared to 96.79% for Dermazin, 87.05% for Ulva lactuca, 90.23% for the mixture, and only 36.44% for the negative controls. A well-formed basement membrane, well-differentiated epithelial cells, and regular thick keratinocytes lining the surface of the epidermal cells accompanied wound healing in rats treated with ESM, which was significantly better than in control rats.
CONCLUSIONS: Ground hen ESM powder, a low-cost effective biomaterial, is better than Ulva lactuca or their mixture for preventing tissue damage and promoting diabetic wound healing, in addition to various biomedical applications.