本文旨在探讨AOL-s00215g415(Aog415)基因的功能,编码线虫诱捕真菌模型菌株A.寡孢子中铁载体的合成,为了了解铁载体生物合成与线虫诱捕活性之间的关系。经过序列分析,确定Aog415是铁载体合成NRPS。然后,该基因的产物被鉴定为异羟肟酸铁载体去铁erriferrichrome,使用质谱分析。当与WT菌株相比时,Aog415敲除菌株在发酵液中的铁载体含量降低了60%。此外,捕食环的数量减少了23.21%,而孢子产量增加了37.34%。Aog415的缺失不影响寡孢子囊在不同营养培养基中的生长。代谢组学分析显示,脂质代谢相关途径是Aog415破坏的主要目标。与WT相比,甘油磷脂水平的显著降低,在突变中观察到糖脂。脂肪酰基和氨基酸样分子的代谢改变被显著破坏。Aog415的敲除损害了异羟肟酸铁载体的生物合成,重塑了寡头孢菌中脂肪酸的流动,主要是重新编程细胞膜脂质代谢。Desferriferrichrome,异羟肟酸铁载体影响生长,通过调节铁的摄入和细胞膜稳态,使少孢菌的代谢和线虫捕获能力。我们的研究揭示了铁载体对生长和线虫捕获能力的重要贡献,并构建了铁载体生物合成之间的关系,寡孢菌的脂质代谢和线虫捕获活性,这为基于线虫诱捕真菌的线虫生物防治剂的开发提供了新的见解。
The objective of this paper is to explore the function of the AOL-s00215g415 (Aog415) gene, which encodes for the synthesis of siderophore in the nematode trapping fungal model strain A. oligospora, in order to understand the relationship between siderophore biosynthesis and nematode trapping activity. After a through sequence analysis, it was determined that Aog415 is a siderophore-synthesizing NRPS. The product of this gene was then identified to be the hydroxamate siderophore
desferriferrichrome, using mass spectrometry analysis. When compared to the WT strains, the Aog415 knockout strain exhibited a 60% decrease in siderophore content in fermentation broth. Additionally, the number of predatory rings of decreased by 23.21%, while the spore yield increased by 37.34%. The deletion of Aog415 did not affect the growth of A. oligospora in diverse nutrient medium. Lipid metabolism-related pathways were the primary targets of Aog415 disruption as revealed by the metabolomic analysis. In comparison to the WT, a significant reduction in the levels of glycerophospholipids, and glycolipids was observed in the mutation. The metabolic alteration in fatty acyls and amino acid-like molecules were significantly disrupted. The knockout of Aog415 impaired the biosynthesis of the hydroxamate siderophore
desferriferrichrome, remodeled the flow of fatty acid in A. oligospora, and mainly reprogrammed the membrane lipid metabolism in cells.
Desferriferrichrome, a hydroxamate siderophore affects the growth, metabolism and nematode trapping ability of A. oligospora by regulating iron intake and cell membrane homeostasis. Our study uncovered the significant contribution of siderophores to the growth and nematode trapping ability and constructed the relationship among siderophores biosynthesis, lipid metabolism and nematode trapping activity of A. oligospora, which provides a new insight for the development of nematode biocontrol agents based on nematode trapping fungi.