■人类毛囊经历循环阶段-生长期,capagen,和静止期-贯穿其一生。已经研究了这种循环转变作为治疗脱发的目标。最近,研究了人毛囊中自噬的抑制与促发展阶段之间的相关性。然而,自噬在人毛乳头细胞(hDPCs)中的作用,参与毛囊的发育和生长,不知道。我们假设在抑制自噬时毛发降解阶段的加速是由于hDPCs中Wnt/β-catenin信号的下调,人参提取物的成分可以增加hDPCs的自噬通量。
■我们使用3-甲基腺嘌呤(3-MA)产生了自噬抑制条件,一种特定的自噬抑制剂,并使用荧光素酶报告基因分析研究了Wnt/β-catenin信号传导的调节,qRT-PCR,和蛋白质印迹分析。此外,用人参皂苷Re和3-MA共混细胞,研究了它们在抑制自噬体形成中的作用。
■我们发现未受刺激的生长期毛乳头区域表达自噬标记,LC3.用3-MA处理hDPC后,Wnt相关基因的转录和β-catenin的核易位减少。此外,人参皂苷Re和3-MA联合治疗通过恢复自噬改变了Wnt活性和毛发周期。
■我们的结果表明,hDPCs中的自噬抑制通过下调Wnt/β-catenin信号传导加速了启动期。此外,人参皂苷Re,增加了hDPCs中的自噬,可用于减少自噬异常抑制引起的脱发。
UNASSIGNED: The human hair follicle undergoes cyclic phases-anagen, catagen, and telogen-throughout its lifetime. This cyclic transition has been studied as a target for treating hair loss. Recently, correlation between the inhibition of autophagy and acceleration of the catagen phase in human hair follicles was investigated. However, the role of autophagy in human dermal papilla cells (hDPCs), which is involved in the development and growth of hair follicles, is not known. We hypothesized that acceleration of hair catagen phase upon inhibition of autophagy is due to the downregulation of Wnt/β-catenin signaling in hDPCs, and that components of Panax ginseng extract can increase the autophagic flux in hDPCs.
UNASSIGNED: We generated an autophagy-inhibited condition using 3-methyladenine (3-MA), a specific autophagy inhibitor, and investigated the regulation of Wnt/β-catenin signaling using the luciferase reporter assay, qRT-PCR, and western blot analysis. In addition, cells were cotreated with ginsenoside Re and 3-MA and their roles in inhibiting autophagosome formation were investigated.
UNASSIGNED: We found that the unstimulated anagen phase dermal papilla region expressed the autophagy marker, LC3. Transcription of Wnt-related genes and nuclear translocation of β-catenin were reduced after treatment of hDPCs with 3-MA. In addition, treatment with the combination of ginsenoside Re and 3-MA changed the Wnt activity and hair cycle by restoring autophagy.
UNASSIGNED: Our results suggest that autophagy inhibition in hDPCs accelerates the catagen phase by downregulating Wnt/β-catenin signaling. Furthermore, ginsenoside Re, which increased autophagy in hDPCs, could be useful for reducing hair loss caused by abnormal inhibition of autophagy.