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Abstract：
OBJECTIVE: Dermal papilla (DP) stem cells are known for their remarkable regenerative capacity, making them a valuable model for assessing the effects of natural products on cellular processes, including stemness, and autophagy.
METHODS: Autophagy and stemness characteristics were assessed using real-time RT-PCR to analyze mRNA levels, along with immunofluorescence and western blot techniques for protein level evaluation.
RESULTS: Butterfly Pea, Emblica Fruits, Kaffir Lime, and Thunbergia Laurifolia extracts induced autophagy in DP cells. Kaffir Lime-treated cells exhibited increase in the OCT4, NANOG, and SOX2 mRNA (6-, 5, and 5.5-fold, respectively), and protein levels (4-, 3-, and 1.5-fold, respectively). All extracts activated the survival protein kinase B (Akt) in DP cells.
CONCLUSIONS: Natural products are a promising source for promoting hair growth by rejuvenating hair stem cells.
METHODS: Autophagy and stemness characteristics were assessed using real-time RT-PCR to analyze mRNA levels, along with immunofluorescence and western blot techniques for protein level evaluation.
RESULTS: Butterfly Pea, Emblica Fruits, Kaffir Lime, and Thunbergia Laurifolia extracts induced autophagy in DP cells. Kaffir Lime-treated cells exhibited increase in the OCT4, NANOG, and SOX2 mRNA (6-, 5, and 5.5-fold, respectively), and protein levels (4-, 3-, and 1.5-fold, respectively). All extracts activated the survival protein kinase B (Akt) in DP cells.
CONCLUSIONS: Natural products are a promising source for promoting hair growth by rejuvenating hair stem cells.
摘要：
目的：毛乳头（DP）干细胞以其非凡的再生能力而闻名，使它们成为评估天然产物对细胞过程影响的有价值的模型，包括干性，和自噬。
方法：使用实时RT-PCR评估自噬和干性特征，以分析mRNA水平，以及免疫荧光和蛋白质印迹技术用于蛋白质水平评估。
结果：蝴蝶豌豆，Emblica水果，KaffirLime,黄芩提取物诱导DP细胞自噬。Kaffir石灰处理的细胞在OCT4，NANOG，和SOX2mRNA(6-，5，5.5倍，分别)，和蛋白质水平（4-，3-,和1.5倍，分别)。所有提取物激活DP细胞中的存活蛋白激酶B(Akt)。
结论：天然产物是通过再生毛发干细胞促进毛发生长的有希望的来源。
方法：使用实时RT-PCR评估自噬和干性特征，以分析mRNA水平，以及免疫荧光和蛋白质印迹技术用于蛋白质水平评估。
结果：蝴蝶豌豆，Emblica水果，KaffirLime,黄芩提取物诱导DP细胞自噬。Kaffir石灰处理的细胞在OCT4，NANOG，和SOX2mRNA(6-，5，5.5倍，分别)，和蛋白质水平（4-，3-,和1.5倍，分别)。所有提取物激活DP细胞中的存活蛋白激酶B(Akt)。
结论：天然产物是通过再生毛发干细胞促进毛发生长的有希望的来源。
