背景:益生菌必须能够承受胃肠系统的苛刻环境,才能粘附在肠上皮上,促进健康益处。益生菌的使用可以预防或减轻对健康有有害影响的生态失调的影响,促进抗炎,免疫调节,和抗氧化作用。
目的:本研究的目的是制备含有用20%Acryl-EzeII®或Opadry®肠溶聚合物包衣的发酵乳杆菌LF-G89的片剂。
方法:在酸性和碱性pH条件下评估片剂的溶出度,并将溶解介质的等分试样铺板以计数菌落形成单位(CFU)。游离益生菌对1.0、2.0、3.0和4.0的pH水平以及胃蛋白酶的耐受性,胰酶,和胆汁盐,被评估。
结果:在经受pH1.2酸性阶段45分钟后,益生菌从包衣的片剂中释放。在基础阶段,Acry-EzeII®聚合物的释放更高。随着时间的推移,pH1.0至4.0的游离益生菌的CFU以及胃蛋白酶的数量减少,表明细胞死亡。相反,随着时间的推移,胰酶和胆汁盐的CFU增加,证明发酵乳杆菌对水解酶的抗性。
结论:两种涂层聚合物都能够承受酸步骤,可能确保益生菌在小肠中的释放,促进殖民。肠溶材料包衣是一种简单有效的过程,可以增加益生菌的存活率,提供了一个有希望的替代方案,以减轻菌群失调过程的负面影响。
BACKGROUND: Probiotics must be able to withstand the demanding environment of the gastrointestinal system to adhere to the intestinal epithelium, promoting health benefits. The use of probiotics can prevent or attenuate the effects of dysbiosis that have a deleterious effect on health, promoting anti-inflammatory, immunomodulatory, and antioxidant effects.
OBJECTIVE: The aim of the study was to prepare tablets containing Lactobacillus fermentum LF-G89 coated with 20% Acryl-Eze II® or Opadry® enteric polymers.
METHODS: Tablet dissolution was evaluated under acidic and basic pH conditions, and aliquots of the dissolution medium were plated to count the Colony-forming Units (CFU). The free probiotic\'s tolerance to pH levels of 1.0, 2.0, 3.0, and 4.0, as well as to pepsin, pancreatin, and bile salts, was assessed.
RESULTS: The probiotic was released from tablets coated after they withstood the pH 1.2 acid stage for 45 minutes. The release was higher with the Acry-Eze II® polymer in the basic stage. The amount of CFU of free probiotics at pH 1.0 to 4.0 as well as pepsin reduced over time, indicating cell death. Conversely, the CFU over time with pancreatin and bile salts increased, demonstrating the resistance of L. fermentum to these conditions due to hydrolases.
CONCLUSIONS: Both coating polymers were able to withstand the acid step, likely ensuring the release of the probiotic in the small intestine, promoting colonization. Coating with enteric material is a simple and effective process to increase the survival of probiotics, offering a promising alternative to mitigate the negative effects of the dysbiosis process.