未经证实:小鼠模型是原发性干燥综合征(pSS)研究的基础。然而,小鼠和人类在唾液腺(SG)免疫细胞中的比较深度仍然有限.
UNASSIGNED:从基因表达综合数据库下载来自正常受试者和pSS患者的SGs的基因表达谱。然后通过估计RNA转录本的相对亚群(CIBERSORT),通过细胞类型鉴定来评估浸润免疫细胞亚群的比例。使用SG蛋白成功构建了实验性干燥综合征(ESS)小鼠模型。基于小鼠SG组织RNA-Seq数据,使用seq-ImmuCC模型定量分析pSS患者和小鼠模型SG组织中10种免疫细胞的组成比.
UNASSIGNED:使用CIBERSORT反卷积方法计算并获得了31个人类数据样本。免疫细胞浸润结果显示,与正常人SG组织相比,γδT细胞的含量与幼稚CD4+T细胞有显著差异,而浆细胞含量下降。主成分分析显示pSS患者与正常人的免疫细胞浸润存在差异。同时,用于ESS模型小鼠数据分析,我们发现巨噬细胞的比例增加了,而CD4+T细胞的比例,B细胞,单核细胞减少。此外,我们发现单核细胞的比例下降,而在pSS患者和模型小鼠的SG组织中巨噬细胞的比例增加。CD4+T细胞浸润,CD8+T,B细胞也表现出一些差异。
UNASSIGNED:我们全面分析了pSS患者和模型小鼠的SG免疫浸润。我们在免疫细胞水平上证明了小鼠和人类免疫系统的保守和不保守方面,以帮助解释干燥综合征发展过程中免疫机制的主要调节。
UNASSIGNED: Mouse models are the basis for primary Sjögren\'s syndrome (pSS) research. However, the depth of comparisons between mice and humans in salivary gland (SG) immune cells remains limited.
UNASSIGNED: The gene expression profiles of SGs from normal subjects and pSS patients were downloaded from the Gene Expression Comprehensive Database. The proportion of infiltrating immune cell subsets was then assessed by cell type identification by estimating relative subsets of RNA transcripts (CIBERSORT). An experimental Sjögren\'s syndrome (ESS) mouse model was successfully constructed using SG protein. Based on mouse SG tissue RNA-Seq data, the seq-ImmuCC model was used to quantitatively analyze the compositional ratios of 10 immune cells in pSS patients and mouse model SG tissues.
UNASSIGNED: Computed and obtained 31 human data samples using the CIBERSORT deconvolution method. The immune cell infiltration results showed that, compared to normal human SG tissue, the content of gamma delta T cells was significantly different from naive CD4+ T cells and significantly increased, while the plasma cell content decreased. Principal component analysis indicated differences in immune cell infiltration between pSS patients and normal subjects. Meanwhile, for ESS model mouse data analysis, we found that the proportion of macrophages increased, while the proportion of CD4+ T cells, B cells, and monocytes decreased. Furthermore, we found that the proportion of monocytes was decreased, while the proportion of macrophages was increased in the SG tissues of pSS patients and model mice. The infiltration of CD4+ T, CD8+ T, and B cells also showed some differences.
UNASSIGNED: We comprehensively analyzed SG immune infiltration in pSS patients and model mice. We demonstrated conserved and nonconserved aspects of the immune system in mice and humans at the level of immune cells to help explain the primary regulation of immune mechanisms during the development of Sjögren\'s syndrome.