已知与栽培作物相关的根瘤菌通过各种间接或直接机制刺激植物生长。近年来,植物生长促进/促进根际细菌的宿主列表已扩展到包括豆类,大麦,棉花,玉米,大米,蔬菜,花生,大米,小麦,和几种种植园作物。然而,根瘤菌与有机茶园和常规茶园茶树的相互作用知之甚少。在本研究中,从位于孟加拉北部的14个茶园中分离出与茶根际相关的根际细菌物种,印度。总的来说,在实验室条件下,评估了从收集的土壤样品中分离出的16种根际细菌分离株的拮抗和植物生长促进/促进活性。基于16SrRNA基因测序的分子表征显示,芽孢杆菌属占5种,其次是假单胞菌属2种。有趣的是,只有一种分离物与放线菌有关,即,巴克微杆菌。在16个分离株中,分离出的枯草芽孢杆菌OKAKP01,枯草芽孢杆菌BNLG01,B.paramycoidesBOK01,M.barkeriBPATH02和嗜麦芽窄食单胞菌BSEY01对茄病镰刀菌的生长抑制作用最高(68.2至72.8%),假雌蕊(71.1至85.6%),和维exasidium(67.4至78.3%)导致相应的镰刀菌枯萎,灰色枯萎病,和茶树的水疱疫病。Further,这五个分离株还具有显著更大的抗真菌(铁载体生产者,蛋白酶,几丁质酶,和纤维素酶活性)和植物生长促进/促进(吲哚-3-乙酸生产,ACC脱氨酶,氨,和磷酸盐溶解)在其他11种根瘤菌分离物中的性状。因此,选择这五个根瘤菌的分离株是因为它们在苗圃条件下对茶树植物的植物生长促进/促进活性。苗圃实验的结果表明,与对照相比,这五个根际细菌显着提高了茶树的生长速率。因此,这项研究表明,这些根瘤菌可用于配制生物农药和生物肥料,可用于可持续茶叶种植,以改善作物健康,减少疾病发作。
Rhizobacteria associated with cultivated crops are known to stimulate plant growth through various indirect or direct mechanisms. In recent years, the host list of plant growth promotion/promoting rhizobacteria has expanded to include bean, barley, cotton, maize, rice, vegetables, peanut, rice, wheat, and several plantation crops. However, interaction of rhizobacteria with tea plants of organic and conventional tea gardens is poorly understood. In the present study, rhizobacterial species associated with tea rhizosphere were isolated from 14 tea gardens located in North Bengal, India. In total, 16 rhizobacterial isolates isolated from collected soil samples were assessed for antagonistic and plant growth promotion/promoting activity under laboratory conditions. Molecular characterization based on sequencing of 16S rRNA gene revealed dominance of Bacillus with five species followed by Pseudomonas with two species. Interestingly, only one isolate was affiliated with actinobacteria, i.e., Microbacterium barkeri. Out of 16 isolates, isolates Bacillus subtilis OKAKP01, B. subtilis BNLG01, B. paramycoides BOK01, M. barkeri BPATH02, and Stenotrophomonas maltophilia BSEY01 showed highest growth inhibition against Fusarium solani (68.2 to 72.8%), Pseudopestalotiopsis theae (71.1 to 85.6%), and Exobasidium vexans (67.4 to 78.3%) causing respective Fusarium dieback, gray blight, and blister blight diseases in tea crop. Further, these five isolates also possessed significantly greater antifungal (siderophore producer, protease, chitinase, and cellulase activity) and plant growth promotion/promoting (indole-3-acetic acid production, ACC deaminase, ammonia, and phosphate solubilization) traits over other eleven rhizobacterial isolates. Therefore, these five isolates of rhizobacteria were chosen for their plant growth promotion/promoting activity on tea plants in nursery conditions. Results from nursery experiments revealed that these five rhizobacteria significantly improved growth rates of tea plants compared with the control. Therefore, this study suggests that these rhizobacteria could be used to formulate biopesticides and biofertilizers, which could be applied to sustainable tea cultivation to improve crop health and reduce disease attack.