The development of food technologies at the present stage is aimed at expanding the range of food raw materials, including alternative food sources. One of such sources is meat derived from in vitro stem cells or cultured meat. The stages of in vitro meat production could be divided into four blocks: preparation of raw materials, cultivation of cells in a nutrient medium, forming the final product and preparing meat for sale to the consumer. The cultured meat production process must be accompanied by the improvement, implementation and maintenance of procedures based on HACCP principles. However, the developed and approved HACCP system for the cultured meat production hasn\'t been found in the scientific literature. Given the prospects for cultured meat production, the development HACCP system for this area is feasible. In this regard, it is advisable to identify critical control points in production and identify the potential hazards of cultured meat to consumers for subsequent risk assessment. The aim of the study was to identify potential health hazards and analyze key control points in cultured meat production. Material and methods. Previously conducted studies on in vitro meat cultivation technologies, as well as Russian regulatory and technical documentation were used as initial data on the production processes of cultured meat and the risk associated with its production. The method of studying and critical analyzing relevant scientific sources devoted to the safety of cultured meat was applied. In total, more than 120 sources were studied, from which 30 relevant ones were selected. Results. The potential hazards associated with the consumption of cultured meat are due to physical, chemical and biological factors. In addition, the occurrence of allergic reactions is identified as a danger factor. An imbalance of amino acids in the diet may occur as a result of the consumption of novel food in a significant proportion (>50% of meat consumption). Ten critical control points have been identified, each of which is characterized by the action of potential hazard factors of a different nature. It has been established that the number of critical control points is the same at all stages of production, except for the formation of the final product. The characteristics of hazard factors, as well as information about the critical control points of these factors\' action, determine the possibility of assessing the potential hazard (and further risk assessment) and choosing measures to manage them, which meets the requirements of GOST R ISO 22000-2019. Conclusion. Each of the identified types of hazard is not specific to a particular stage of in vitro meat production and can effect negatively at several critical control points. When organizing control (monitoring) of the safety of new type food, one should be guided by the Technical Regulations of the Custom Union \"On the Safety of Food Products\", according to which the manufacturer of foods (including novel foods) must develop, implement and comply with procedures based on the HACCP. The use of HACCP is relevant under inappropriate risk for consumer health. To assess the risk level, exposure assessment (a key step in risk assessment) should be carried out for each type of hazard factor. For these purposes, it is necessary to identify the potential risk group and determine the scenarios of cultured meat\'s consumption.

Human health hazards appear in wine production. Wineries have implemented food safety management systems to control food hazards through Hazard Analysis Critical Control Point (HACCP). Wine-making industry applies HACCP by evaluating Critical Control Points (CCPs). One of the CCPs that exhibits inadequate control is the potential contamination risk of arsenic, cadmium, and lead throughout the winemaking procedure. Wineries performance level about controlling CCPs related to contamination risk by arsenic, cadmium and lead in the winemaking were analyzed. A sixteen-question questionnaire was made to achieve this research. Three indicators were calculated for training, legislation, and analysis performance components in CCPs control. Results revealed that wineries fault in analysis and legislation components. Identification and updating of legislation about As, Cd and Pb contamination risk is in starting performance level for wineries that produce less than 250,000 L/year wineries. Analysis performance level is even lower than legislation. Only one out of every three wineries possess information regarding the concentrations of arsenic, cadmium, and lead in the soils of vineyards where grapes are cultivated. Furthermore, the availability of data on their available concentrations in the soil solution is even more limited. Those wineries that controlled As, Cd and Pb concentrations make it according to official recommendations using techniques based on atomic absorption spectrometry. However, there is a lack of this spectrometry equipment in the wineries own laboratories.

Patulin (PAT) is a toxic secondary metabolite produced by Aspergillus sp. and Penicillium sp., which acts as a contaminant of most apples and their products. The internationally recognized HACCP system is selected as the theoretical basis to more effectively reduce the PAT in apple juice concentrate (AJC). Through field investigation of apple juice concentrate (AJC) production enterprises, we collected 117 samples from 13 steps of AJC production, including whole apple, apple pulp, and apple juice. PAT contents were analyzed via high-performance liquid chromatography (HPLC) and compared with samples from the different production processes. The result demonstrated that the PAT content was significantly (p < 0.05) influenced by five processes, receipt of raw apples, sorting of raw apples, adsorption step, pasteurization, and aseptic filling. These processes were determined as the CCPs. Monitoring systems for maintaining CCPs within acceptable limits were established, and corrective actions were proposed in case a CCP was surpassed. Based on the above-identified CCPs, critical limits, and control methods (corrective actions), a HACCP plan related to the production process of AJC was established. This study provided important guidance for juice manufacturers wishing to effectively control the PAT content in their products.

UNASSIGNED: Enterohemorrhagic Escherichia coli (O157:H7) is the primary cause of bloody diarrhea or hemorrhagic colitis. The study was carried out with to determine the epidemiology and antimicrobial resistance pattern of E. coli O157:H7 along the dairy supply chains in Akaki Kaliti sub-city of Addis Ababa, Bishoftu and Sululta towns of central Ethiopia.
UNASSIGNED: A cross-sectional study design with random sampling methods was employed. Thus, a total of 450 raw cow milk (294), milker hand swab (65) and water (91) samples were collected from dairy farms, milk collection centers and Cafeterias and processed according to the standards to isolate and identify E. coli O157:H7. The samples were initially enriched in buffered peptone water, then plated onto Sorbitol MacConkey agar. Consequently, the suspected non-sorbitol fermenting colonies were confirmed as E. coli biochemically and serological test using latex agglutination tests.
UNASSIGNED: Out of the total 450 samples examined, 6.0% were found to be contaminated by E. coli O157:H7. Accordingly, 9.89% of water, 9.23% of milker hand swab and 4.08% of raw milk samples were contaminated by the pathogen. Furthermore, the prevalence of E. coli O157:H7 was 7.79%, 6.21% and 3.97% in Akaki kaliti sub-city, Sululta and Bishoftu towns, respectively. The result of Fisher exact analysis revealed a significant difference observed (p < 0.05) between the occurrence of the pathogen and the source of sample, sources of water used, sampled material and type of containers. The study also revealed that varying level of resistance of E. coli O157:H7 isolates against nine antimicrobial discs tested and 100% (n = 27) of the isolates showed multidrug-resistance comprising from two up to seven antimicrobial drugs.
UNASSIGNED: In conclusion, this study has indicated the occurrence of E. coli O157:H7 and its multiple drug-resistant profiles in milk samples along the dairy supply chains and its risk to public health and food safety. Therefore, proper hygienic practices from dairy farms to fork and rational drug usage are recommended.

Food manufacturers are required to obtain scientific and technical evidence that a control measure or combination of control measures is capable of reducing a significant hazard to an acceptable level that does not pose a public health risk under normal conditions of distribution and storage. A validation study provides evidence that a control measure is capable of controlling the identified hazard under a worst-case scenario for process and product parameters tested. It also defines the critical parameters that must be controlled, monitored, and verified during processing. This review document is intended as guidance for the food industry to support appropriate validation studies, and aims to limit methodological discrepancies in validation studies that can occur among food safety professionals, consultants, and third-party laboratories. The document describes product and process factors that are essential when designing a validation study, and gives selection criteria for identifying an appropriate target pathogen or surrogate organism for a food product and process validation. Guidance is provided for approaches to evaluate available microbiological data for the target pathogen or surrogate organism in the product type of interest that can serve as part of the weight of evidence to support a validation study. The document intends to help food manufacturers, processors, and food safety professionals to better understand, plan, and perform validation studies by offering an overview of the choices and key technical elements of a validation plan, the necessary preparations including assembling the validation team and establishing prerequisite programs, and the elements of a validation report.

OBJECTIVE: To analyze the results of international proficiency tests for aflatoxin determination and to identify the requirement of quality assurance and control in mycotoxin analysis.
METHODS: By the use of Z-scores, the results of aflatoxins from international proficiency tests were analyzed and the critical control points in experimental operation were discussed.
RESULTS: The absolute values of the Z-scores of aflatoxins were less than 2, indicating that the results from the laboratory were satisfactory. The sampling and testing method had very important effects on the detection results.
CONCLUSIONS: The results of the international proficiency tests of aflatoxins suggested that to strengthen the critical control points in the experimental operation can effectively improve the accuracy and reliability of the determination results.

Following identification of some cases of diarrhea from persons who either sought treatment at a health clinic that served two townships near a large city in Zambia or got water from a deep protected well in one of the townships, hazard analyses were done of food preparation and storage practices at 17 homes. Samples of foods at various stages of preparation, foods held overnight, and drinking water were collected from the homes of the ill persons and were tested for common foodborne pathogens and indicator organisms. Salmonella was isolated from a sample of leftover kapenta (cooked dried minnows). Thermotolerant coliforms and Escherichia coli were isolated from water from shallow wells and a treated community supply. Although thermtolerant coliforms were not recovered from the protected well site, they were isolated from a sample of water collected in a home that used this supply. Several leftover foods, however, contained much larger populations of thermotolerant coliforms and larger populations of aerobic mesophilic organisms than the water. Furthermore, leftover nshima (boiled and whipped corn meal) and porridge contained large populations (>105) of Bacillus cereus per gram. Foods during cooking attained temperatures that would have been lethal to vegetative cells of foodborne pathogens. After cooking, they were subjected to time-temperature abuse during holding until eaten or while held overnight.

Hazard analyses were conducted at several cooked food vending operations in a large city in Zambia, near a downtown bus park and at a large market. Samples of raw, processed, and cooked foods sold on streets or by small food shops were collected and tested for common foodborne pathogens and indicator organisms. Results showed that some raw foods (ground meat, chicken, chicken intestine) or processed foods (dried minnows; kapenta) were contaminated by salmonellae or contained high populations of Staphylococcus aureus (pasteurized milk) or Bacillus cereus (caterpillars). Cooking usually gave time-temperature exposures that would have been lethal for vegetative forms of foodborne pathogens. Holding of foods other than nshima often provided time-temperature exposures conducive to microbial growth, particularly in foods held overnight. Large populations of aerobic mesophilic organisms, thermotolerant coliform bacteria, and sometimes Escherichia coli were recovered from these foods. Ten million Clostridium peifringens per gram were isolated from a sample of leftover beef stew. Large populations (> 105) of S. aureus were recovered from a sample of leftover chicken, and large populations of B. cereus (> 107) were isolated from leftover rice. Time-temperature exposures during reheating had variable effects in terms of killing the microorganisms that germinated from surviving spores or that reached the foods after cooking, but heat-stable toxins would not have been inactivated.

Decision trees have been used as an aid to selection of critical control points as part of the development of hazard analysis critical control point (HACCP) systems. The background for those in existence is described. Another decision-tree approach that follows the logic in the IAMFES manual Procedures to Implement the Hazard Analysis Critical Control Point Approach is presented. It takes into consideration impending hazards, the effect of actions exercised at the operation in question, whether control actions should be taken at this or subsequent operations and whether the CCP will be monitored and corrections made. Further, guidelines are given for selecting an operation as a critical control point. A decision tree to aid in the evaluation of risks is presented, considering whether illness will result, the severity of the illness and the likely occurrence of this outcome, based upon epidemiologic or challenge study of related events. These decision trees provide additional tools to aid in the development of HACCP systems.

A Quality Management Framework to improve quality and harmonization of Total Diet Study practices in Europe was developed within the TDS-Exposure Project. Seventeen processes were identified and hazards, Critical Control Points and associated preventive and corrective measures described. The Total Diet Study process was summarized in a flowchart divided into planning and practical (sample collection, preparation and analysis; risk assessment analysis and publication) phases. Standard Operating Procedures were developed and implemented in pilot studies in five organizations. The flowchart was used to develop a quality framework for Total Diet Studies that could be included in formal quality management systems. Pilot studies operated by four project partners were visited by project assessors who reviewed implementation of the proposed framework and identified areas that could be improved. The quality framework developed can be the starting point for any Total Diet Study centre and can be used within existing formal quality management approaches.