目的:各种组织中线粒体功能的改变是太空飞行引起的物理调节的关键决定因素。与组织活检相比,血细胞生物能量学有望成为一种系统性和更容易获得的生物标志物,在头下倾斜卧床休息(HDTBR)期间进行了评估,建立的地面模拟,用于航天引起的人类生理变化。更具体地说,本研究探讨了HDTBR和运动对策对外周血单个核细胞(PBMC)线粒体呼吸的影响。
方法:我们对24名健康参与者进行了严格的30天HDTBR方案。对照组(n=12)仅接受HDTBR,而对策组(n=12)进行常规仰卧位自行车运动,然后在运动后进行静脉闭塞大腿袖带6小时。我们在卧床休息前14天评估了常规血液参数,通过高分辨率呼吸测定PBMC的呼吸能力,在卧床休息前2天和第30天的柠檬酸合酶活性。我们通过流式细胞术确认了PBMC组成。
结果:对策组PBMC最大氧化磷酸化能力(OXPHOS)的变化增加了11%,而对照组下降了10%(p=0.04)。OXPHOS的限制仅在对照组中增加,而其他呼吸状态不受任何干预的影响。相关分析显示白细胞之间呈正相关,淋巴细胞,两组中都有PBMC生物能量学的嗜碱性粒细胞。
结论:这项研究表明,有规律的运动对策对PBMC线粒体功能有积极影响,证实了血细胞生物能学在人类太空飞行中的潜在应用。
Altered mitochondrial function across various tissues is a key determinant of spaceflight-induced physical deconditioning. In comparison to tissue biopsies, blood cell bioenergetics holds promise as a systemic and more readily accessible biomarker, which was evaluated during head-down tilt bed rest (HDTBR), an established ground-based analog for spaceflight-induced physiological changes in humans. More specifically, this study explored the effects of HDTBR and an exercise
countermeasure on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs).
We subjected 24 healthy participants to a strict 30-day HDTBR protocol. The control group (n = 12) underwent HDTBR only, while the
countermeasure group (n = 12) engaged in regular supine cycling exercise followed by veno-occlusive thigh cuffs post-exercise for 6 h. We assessed routine blood parameters 14 days before bed rest, the respiratory capacity of PBMCs via high-resolution respirometry, and citrate synthase activity 2 days before and at day 30 of bed rest. We confirmed PBMC composition by flow cytometry.
The change of the PBMC maximal oxidative phosphorylation capacity (OXPHOS) amounted to an 11% increase in the
countermeasure group, while it decreased by 10% in the control group (p = 0.04). The limitation of OXPHOS increased in control only while other respiratory states were not affected by either intervention. Correlation analysis revealed positive associations between white blood cells, lymphocytes, and basophils with PBMC bioenergetics in both groups.
This study reveals that a regular exercise
countermeasure has a positive impact on PBMC mitochondrial function, confirming the potential application of blood cell bioenergetics for human spaceflight.