为揭示水性铜胁迫对基因表达变化的影响,分子途径,和纤毛鼻的生理功能,幼鱼平均分为两个实验组,铜含量分别为1.61±0.03mg/L(铜暴露组)和0mg/L(对照组),分别。4小时后,收集g组织样本进行转录物测序分析,从铜处理组(Cu)和对照组(C)构建两个文库,并使用Illumina测序技术进行测序。结果表明,从每个文库中获得了大约40.2-46.0M的干净读数,唯一映射转录本的百分比在80.57至84.93%之间。在水性铜胁迫下,共鉴定出3915个差异表达基因(DEGs),其中1300个基因上调,2615个基因下调。随机选择12个DEGs进行定量RT-PCR(qRT-PCR)分析,结果证实转录组分析是可靠的。此外,对DEGs进行了基因本体论(GO)和京都基因和基因组百科全书(KEGG)富集分析,结果表明,大多数DEGs参与代谢途径,包括类固醇生物合成,谷胱甘肽代谢,和过氧化物酶体增殖物激活受体(PPAR)信号通路。此外,由于水性铜含量,gsk-3β显著上调,而其他代谢相关基因(Tor,pi3k,lpl,aqp7、fabp3)显著下调。此外,铜暴露组显着降低了一些免疫基因的表达(ifn-γ,stat1、cxcl10和tgf-β),并增强il-1β和tnf-α的表达。总之,这些结果表明,铜导致体内代谢紊乱和能量供应不足,并诱导氧化应激,导致免疫功能下降。
To reveal the effects of waterborne copper stress on gene expression changes, molecular pathways, and physiological functions in Coilia nasus, juvenile fish were equally divided into two experimental groups, and the copper levels were 1.61 ± 0.03 mg/L (copper-exposed group) and 0 mg/L (control group), respectively. After 4 h, gill tissue samples were collected for transcript sequencing analysis, and two libraries were constructed from the copper treatment group (Cu) and the control group (C) and sequenced using Illumina sequencing technology. The results showed that approximately 40.2-46.0 M clean reads were obtained from each library, and the percentage of uniquely mapped transcripts ranged from 80.57 to 84.93%. A total of 3915 differentially expressed genes (DEGs) were identified under waterborne copper stress, among which 1300 genes were up-regulated, and 2615 genes were down-regulated. Twelve DEGs were randomly selected for quantitative RT-PCR (qRT-PCR) analysis, and the results confirmed that the transcriptome analysis was reliable. Furthermore, the DEGs were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and the results showed that most of the DEGs were involved in metabolic pathways, including steroid biosynthesis, glutathione metabolism, and peroxisome proliferator-activated receptor (PPAR) signaling pathways. Furthermore, due to the waterborne copper levels, gsk-3β was significantly up-regulated, while other metabolism-related genes (tor, pi3k, lpl, aqp7, fabp3) were significantly down-regulated. In addition, the copper-exposed group significantly reduced the expression of some immunity genes (ifn-γ, stat1, cxcl10, and tgf-β), and enhanced the expression of il-1β and tnf-α. In summary, these results indicated that copper causes metabolic disorders and insufficient energy supply in the body, and induces oxidative stress, which results in reduced immune functions.