PDF(Pubmed)
Abstract：
In this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1T could highly tolerate Cu2+ up to 10 mM, and the isolates could also grow in a wide range of pH (3-7), and temperature (10-40 °C). Dried biomass of GL1 was able to remove Cu2+ effectively up to 90.49% with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 °C, and incubation time 60 min. Sequence analysis of rDNA indicated this strain was closely related to Rhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2 domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respectively. Based on phylogenetic tree analysis and the biochemical characteristics, the strain appears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is proposed. This study provides us with more information about heavy metal-tolerant yeasts and it may produce a new tool for environmental control and metal recovery operations.
摘要：
在这项研究中,从嘉莱金矿收集的土壤样本中分离出二十五株酵母菌株,越南。其中,一种名为GL1T的分离物可以高度耐受高达10mM的Cu2+，分离物也可以在宽范围的pH（3-7）中生长，和温度（10-40°C）。GL1的干燥生物质能够有效去除高达90.49％的Cu2，在pH6，温度30°C时的最大生物吸附能力为18.1mg/g，和孵育时间60分钟。rDNA序列分析表明，该菌株与粘胶红酵母密切相关，但28SrRNA基因的D1/D2结构域和ITS1-5.8SrRNA基因-ITS2区域序列的核苷酸差异为1.53％和3.46％，分别。基于系统发育树分析和生化特征,该菌株似乎是一种新的红球藻，这个名字叫红景天。11月。是提议的。这项研究为我们提供了有关耐重金属酵母的更多信息，它可能会为环境控制和金属回收操作提供新的工具。
