Thyroid cancer is among the most common endocrine malignancies, necessitating effective surgical interventions. Traditional open cervicotomy has long been the standard approach for thyroidectomy. However, the advent of robotic surgery has introduced new possibilities for minimally invasive procedures with benefits in terms of cosmetic outcomes, enhanced precision, comparable complication rates, and reduced recovery time. This study mainly reviewed the most widely used and well-known robotic thyroidectomy approaches: the transaxillary approach, the bilateral axillo-breast approach, and the transoral approach. This review examines the current status and future potential of robotic surgery in thyroid cancer treatment, comparing its efficacy, safety, and outcomes with those of conventional open cervicotomy. Challenges such as a longer operative time and higher costs exist. Future directions include technological advancements, tele-surgery, single-port surgery, and the integration of artificial intelligence. Robotic surgery holds promise in optimizing patient outcomes in thyroid cancer treatment.

According to the 7th National Population Census, China is experiencing rapid growth of its ageing population, with large spatial disparities in the distribution of older folks in different regions. And yet, scant comparative research has been conducted on the two regions of Zhejiang and Jilin in particular, which differ considerably in economic development but witness nearly the same ageing trend. In response, this article compares Zhejiang, an advanced economic province, with Jilin, with its relatively low level of economic development, to explore the ageing issue and analyse the spatial correlation between older populations and socioeconomic factors. Using the spatiotemporal data analysis and geographical detector approaches, we obtain three significant findings: 1. both provinces have maintained steady rates of increase in ageing; 2. the older populations in Zhejiang and Jilin are mostly concentrated in the provincial capitals and nearby cities with reasonably established economies; and 3. the factors, including local fiscal expenditures, beds in hospitals and nursing homes, and coverage of social security, show a highly similar spatial pattern between older populations in Zhejiang and Jilin. The q-values of all the selected socioeconomic factors in Jilin showed a growth trend, indicating that the spatial correlation between these factors and ageing is strengthening year on year, that is, the resources gained from the socioeconomic development of Jilin have shifted steadily toward old-age services. As a consequence, a vicious circle of the slowing down of the economic growth drives away working forces and quickens the pace of population ageing, is present. From a policy perspective, Jilin province is strongly dependent on state-owned enterprises characterised by institutional rigidity, an inflexible market economy and an under-developed private sector, all of which are profoundly influenced by ageing. The consequence is large population outflows of young people. In contrast, the economy of Zhejiang province is partially decoupled from the ageing trend, so the gap in level of development between its counties has been narrowing. The policy implication here is that Zhejiang represents an active private economy that has coped successfully with ageing by attracting young migrants and developing new forms of development, such as the digital economy.

The intracellular protozoan parasite Babesia gibsoni infects canine erythrocytes and causes babesiosis. The hazards to animal health have increased due to the rise of B. gibsoni infections and medication resistance. However, the lack of high-quality full-genome sequencing sets has expanded the obstacles to the development of pathogeneses, drugs, and vaccines. In this study, the whole genome of B. gibsoni was sequenced, assembled, and annotated. The genomic size of B. gibsoni was 7.94 Mbp in total. Four chromosomes with the size of 0.69 Mb, 2.10 Mb, 2.77 Mb, and 2.38 Mb, respectively, 1 apicoplast (28.4 Kb), and 1 mitochondrion (5.9 Kb) were confirmed. KEGG analysis revealed 2,641 putative proteins enriched on 316 pathways, and GO analysis showed 7,571 annotations of the nuclear genome in total. Synteny analysis showed a high correlation between B. gibsoni and B. bovis. A new divergent point of B. gibsoni occurred around 297.7 million years ago, which was earlier than that of B. bovis, B. ovata, and B. bigemina. Orthology analysis revealed 22 and 32 unique genes compared to several Babesia spp. and apicomplexan species. The metabolic pathways of B.gibsoni were characterized, pointing to a minimal size of the genome. A species-specific secretory protein SA1 and 19 homologous genes were identified. Selected specific proteins, including apetala 2 (AP2) factor, invasion-related proteins BgAMA-1 and BgRON2, and rhoptry function proteins BgWH_04g00700 were predicted, visualized, and modeled. Overall, whole-genome sequencing provided molecular-level support for the diagnosis, prevention, clinical treatment, and further research of B. gibsoni. IMPORTANCE The whole genome of B. gibsoni was first sequenced, annotated, and disclosed. The key part of genome composition, four chromosomes, was comparatively analyzed for the first time. A full-scale phylogeny evolution analysis based on the whole-genome-wide data of B. gibsoni was performed, and a new divergent point on the evolutionary path was revealed. In previous reports, molecular studies were often limited by incomplete genomic data, especially in key areas like life cycle regulation, metabolism, and host-pathogen interaction. With the whole-genome sequencing of B. gibsoni, we provide useful genetic data to encourage the exploration of new terrain and make it feasible to resolve the theoretical and practical problems of babesiosis.

Several assistive technology solutions, targeting the group of Blind and Visually Impaired (BVI), have been proposed in the literature utilizing multi-sensor data fusion techniques. Furthermore, several commercial systems are currently being used in real-life scenarios by BVI individuals. However, given the rate by which new publications are made, the available review studies become quickly outdated. Moreover, there is no comparative study regarding the multi-sensor data fusion techniques between those found in the research literature and those being used in the commercial applications that many BVI individuals trust to complete their everyday activities. The objective of this study is to classify the available multi-sensor data fusion solutions found in the research literature and the commercial applications, conduct a comparative study between the most popular commercial applications (Blindsquare, Lazarillo, Ariadne GPS, Nav by ViaOpta, Seeing Assistant Move) regarding the supported features as well as compare the two most popular ones (Blindsquare and Lazarillo) with the BlindRouteVision application, developed by the authors, from the standpoint of Usability and User Experience (UX) through field testing. The literature review of sensor-fusion solutions highlights the trends of utilizing computer vision and deep learning techniques, the comparison of the commercial applications reveals their features, strengths, and weaknesses while Usability and UX demonstrate that BVI individuals are willing to sacrifice a wealth of features for more reliable navigation.

BACKGROUND: The transcriptional changes around zygotic genome activation (ZGA) in preimplantation embryos are critical for studying mechanisms of embryonic developmental arrest and searching for key transcription factors. However, studies on the transcription profile of porcine ZGA are limited.
RESULTS: In this study, we performed RNA sequencing in porcine in vivo developed (IVV) and somatic cell nuclear transfer (SCNT) embryo at different stages and compared the transcriptional activity of porcine embryos with mouse, bovine and human embryos. The results showed that the transcriptome map of the early porcine embryos was significantly changed at the 4-cell stage, and 5821 differentially expressed genes (DEGs) in SCNT embryos failed to be reprogrammed or activated during ZGA, which mainly enrichment to metabolic pathways. c-MYC was identified as the highest expressed transcription factor during ZGA. By treating with 10,058-F4, an inhibitor of c-MYC, the cleavage rate (38.33 ± 3.4%) and blastocyst rate (23.33 ± 4.3%) of porcine embryos were significantly lower than those of the control group (50.82 ± 2.7% and 34.43 ± 1.9%). Cross-species analysis of transcriptome during ZGA showed that pigs and bovines had the highest similarity coefficient in biological processes. KEGG pathway analysis indicated that there were 10 co-shared pathways in the four species.
CONCLUSIONS: Our results reveal that embryos with impaired developmental competence may be arrested at an early stage of development. c-MYC helps promote ZGA and preimplantation embryonic development in pigs. Pigs and bovines have the highest coefficient of similarity in biological processes during ZGA. This study provides an important reference for further studying the reprogramming regulatory mechanism of porcine embryos during ZGA.

Different flattened bamboo boards will produce different visual effects, which directly determine consumers\' preferences. However, their visual characteristics were unknown. To clarify the visual effects of flattened bamboo boards in different bamboo culms, the visual, physical, and psychological quantities were firstly studied using their quantitative color and glossiness measurements, combined with quantitative semantic differential and statistical methods. Key results revealed that the values of lightness L* and blue-yellow index b* from the base to the top of the bamboo culms tended to decrease gradually, while green-red index a* values exhibited an increasing trend, and glossiness GZL (GZT) showed no significant difference. The L* value of bamboo outer layer (30.18) was smaller than that of the outer (61.90) and the inner (68.68), which had an increasing trend from the outside to the inside of the bamboo culm, while the GZL (GZT) values corresponded to 6.07 (4.66), 4.51 (3.12), and 2.77 (2.55), showing an opposite trend. The a* and b* values present a rise-fall tendency. According to visual psychological assessment, the outer was reflected as an \"artificial-decorative\", \"smooth-warm\", and \"comfort-sophisticated\" feeling; the inner had an \"artificial-practical\", \"smooth-warm\", and \"comfort-sophisticated\" sense; the bamboo outer layer had an \"nature-practical\", \"rough-cold\", and \"sick-primitive\" sense. Furthermore, predictive models for visual psychological quantities were constructed. This work provides a theoretical data basis for furniture design and standard materials application of flattened bamboo boards.

Tilia is a complex genus in the family Malvaceae that has high ecological and economical values. Owing to the lack of sufficient distinguishable morphological and molecular characteristics, interspecific relationships in this genus are not clear. Chloroplast (cp) genomes are small, meanwhile most angiosperms usually undergo matrilineal inheritance. Consequently, they can be used in molecular systematics and phylogenetic analyses. Here, we sequenced and assembled cp genomes from T. endochrysea, T. tomentosa, T. miqueliana, T. americana and T. cordata, and compared them with those of seven previously reported Tilia species. Similar gene contents, gene orders and GC contents existed among the 12 cp genomes, which ranged from 162,564 to 162,855 bp and encoded 113 unique genes. Abundant simple sequence repeats (119-127) and dispersed repeats (97-135) were detected in Tilia cp genomes. In total, 11 hypervariable regions were identified that could be suitable for species identification and phylogenetic studies. A phylogenetic analysis of Malvaceae based on 5 hypervariable genes (matK + ndhF + rpoB + rpoC2+ycf1) revealed that all eight subfamilies were monophyletic groups. Additionally, the genus Tilia was divided into three groups on the basis of all 521 molecular variation loci. The current study provides valuable insights into the genomic evolution of the genus Tilia.

The effects of insecticides on bee health are a topic of intensive research. Although abamectin is toxic to bees, the molecular impact of abamectin needs to be clarified. Here, we found that Apis cerana cerana exhibited a higher mortality rate when exposed to abamectin than Apis mellifera ligustica. In addition, A. cerana cerana had markedly higher numbers of differentially expressed genes (DEGs), differentially expressed proteins (DEPs) and differentially expressed metabolites (DEMs) than A. mellifera ligustica during exposure to abamectin. These results indicate that abamectin exposure exerts stronger effects on A. cerana cerana than on A. mellifera ligustica. In addition, six DEGs, two DEPs and two DEMs overlapped between the two bee species under abamectin exposure; however, some genes or proteins from the zinc finger protein, superoxide dismutase and peroxiredoxin families and the energy metabolism pathway were only unregulated in A. cerana cerana, which indicates a significant difference in the impact of abamectin on the two bee species. Despite these differences, several of the same gene families, such as heat shock proteins, cytochrome P450, odorant-binding proteins and cuticle proteins, and pathways, including the carbohydrate metabolism, immune system, lipid metabolism, amino acid metabolism, sensory system, locomotion and development pathways, were influenced by abamectin exposure in both A. cerana cerana and A. mellifera ligustica. Together, our results indicate that abamectin causes adverse effects on bees and thus poses a risk to bee populations and that abamectin exposure affects A. cerana cerana more strongly than A. mellifera ligustica. These findings improve our understanding of the behavioural and physiological effects of abamectin on bees.

Identification of intercellular signaling changes across multiple single-cell RNA-sequencing (scRNA-seq) datasets as well as how intercellular communications affect intracellular transcription factors (TFs) to regulate target genes is crucial in understanding how distinct cell states respond to evolution, perturbations, and diseases. Here, we first generalized our previously developed tool CellChat, enabling flexible comparison analysis of cell-cell communication networks across any number of scRNA-seq datasets from interrelated biological conditions. This greatly facilitates the ready detection of signaling changes of cell-cell communication in response to any biological perturbations. We then investigated how intercellular communications affect intracellular signaling response by inferring a multiscale signaling network which bridges the intercellular communications at the population level and the cell state-specific intracellular signaling network at the molecular level. The latter is constructed by integrating receptor-TF interactions collected from public databases and TF-target gene regulations inferred from a network-regularized regression model. By applying our approaches to three scRNA-seq datasets from skin development, spinal cord injury, and COVID-19, we demonstrated the capability of our approaches in identifying the predominant signaling changes across conditions and the critical signaling mechanisms regulating target gene expression. Together, our work will facilitate the identification of both intercellular and intracellular dysregulated signaling mechanisms responsible for biological perturbations in diverse tissues.

Polyphyllin I (PPI) and its analogues, including polyphyllin II (PPII), polyphyllin VI (PPVI) and polyphyllin VII (PPVII), are major bioactive compounds isolated from the Chinese herb Chonglou. However, the susceptibilities of PPI and its analogues towards the different cell lines are diversified and the mechanisms are not fully clarified. Thus, the present study aimed to investigate the cytotoxicity of PPI and its analogues on two different cell lines, as well as to explore the underlying mechanisms of these agents via inducing mitochondrial dysfunction. The results showed that PPI and its analogues were cytotoxic agents towards both A549 and HT-29 cells, with IC50 values ranged from 1.0 to 4.5 μmol/L. Further investigations demonstrated that they decreased the mitochondrial membrane potentials of both A549 and HT-29 cells in a dose-dependent manner. Among all tested compounds, PPVI and PPI induced the most obvious changes in Ca2+ haemostasis in these two cell lines. In addition, they could induce the accumulation of ROS in cells and down-regulated the Bcl-2 expression, up-regulated the Bax expression and induced the activity of cleaved caspase-3 in cells. Collectively, our findings clearly demonstrated the cytotoxic differences and mechanisms of PPI and its analogues induced cell apoptosis and could partially explain the anticancer effects of these natural constituents in Chonglou.