■胶质母细胞瘤(IV级)是成人中最具侵袭性的原发性脑肿瘤,由于其高度侵略性,代表了最大的治疗挑战之一。在这项研究中,我们研究了毫米波对直接来源于患者肿瘤的三维胶质母细胞瘤器官的影响.我们的目标是在对抗这种具有挑战性的疾病的斗争中探索新的治疗可能性。
■曝光设置是在内部精心开发的,我们采用了全面的剂量测定方法,数值和实验相结合的方法。生物学终点包括全局转录谱分析,以突出可能的失调途径,细胞形态变化分析,和细胞表型表征都是控制胶质母细胞瘤进展的重要参与者。
■我们的结果显示30.5GHz的连续毫米波对细胞增殖和凋亡的显着影响,尽管不影响组成类器官的胶质母细胞瘤细胞的分化状态。令人兴奋的是,当应用0.1W(均方根)的功率水平时,当与化疗药物替莫唑胺联合使用时,我们发现了显着的(统计学上显著的)治疗效果,导致胶质母细胞瘤细胞死亡增加。这些发现为治疗胶质母细胞瘤细胞提供了一个有希望的介入窗口,利用30.5GHzCW暴露的潜在治疗益处。处理期间的温度升高被仔细监测和模拟,具有良好的一致性,证明温度升高对观察到的影响微不足道。通过探索这种创新方法,我们为改善胶质母细胞瘤的未来治疗方法铺平了道路,胶质母细胞瘤至今仍极具挑战性。
UNASSIGNED: Glioblastoma (grade IV) is the most aggressive primary brain tumor in adults, representing one of the biggest therapeutic challenges due to its highly aggressive nature. In this study, we investigated the impact of millimeter waves on tridimensional glioblastoma organoids derived directly from patient tumors. Our goal was to explore novel therapeutic possibilities in the fight against this challenging disease.
UNASSIGNED: The exposure setup was meticulously developed in-house, and we employed a comprehensive dosimetry approach, combining numerical and experimental methods. Biological endpoints included a global transcriptional profiling analysis to highlight possible deregulated pathways, analysis of cell morphological changes, and cell phenotypic characterization which are all important players in the control of glioblastoma progression.
UNASSIGNED: Our results revealed a significant effect of continuous millimeter waves at 30.5 GHz on cell proliferation and apoptosis, although without affecting the differentiation status of glioblastoma cells composing the organoids. Excitingly, when applying a power level of 0.1 W (Root Mean Square), we discovered a remarkable (statistically significant) therapeutic effect when combined with the chemotherapeutic agent Temozolomide, leading to increased glioblastoma cell death. These findings present a promising interventional window for treating glioblastoma cells, harnessing the potential therapeutic benefits of 30.5 GHz CW exposure. Temperature increase during treatments was carefully monitored and simulated with a good agreement, demonstrating a negligible involvement of the temperature elevation for the observed effects. By exploring this innovative approach, we pave the way for improved future treatments of glioblastoma that has remained exceptionally challenging until now.