Abstract：
Pancreatic cancer has a poor prognosis due to its aggressive nature and ability to metastasize at an early stage. Currently, its management is still a challenge because this neoplasm is resistant to conventional treatment approaches, among which is chemo-radiotherapy (CRT), due to the abundant stromal compartment involved in the mechanism of hypoxia. Hyperthermia, among other effects, counteracts hypoxia by promoting blood perfusion and thereby can enhance the therapeutic effect of radiotherapy (RT). Therefore, the establishment of integrated treatments would be a promising strategy for the management of pancreatic carcinoma. Here, the effects of joint radiotherapy/hyperthermia (RT/HT) on optimized chick embryo chorioallantoic membrane (CAM) pancreatic tumor models are investigated. This model enables a thorough assessment of the tumor-arresting effect of the combined approach as well as the quantitative evaluation of hypoxia and cell cycle-associated mechanisms by both gene expression analysis and histology. The analysis of the lower CAM allows to investigate the variation of the metastatic behaviors of the cancer cells associated with the treatments. Overall, this study provides a potentially effective combined strategy for the non-invasive management of pancreatic carcinoma.
摘要：
胰腺癌由于其侵袭性和在早期转移的能力而具有不良预后。目前,它的管理仍然是一个挑战，因为这种肿瘤对传统的治疗方法有抵抗力，其中包括化学放射疗法（CRT），由于缺氧的机制涉及丰富的基质室。高热,在其他影响中，通过促进血液灌注来抵消缺氧，从而增强放疗（RT）的治疗效果。因此,建立综合治疗将是胰腺癌治疗的一个有希望的策略.这里,研究了联合放疗/热疗（RT/HT）对优化的鸡胚绒毛尿囊膜（CAM）胰腺肿瘤模型的影响。该模型可以通过基因表达分析和组织学对组合方法的肿瘤阻滞作用进行全面评估，并定量评估缺氧和细胞周期相关机制。较低CAM的分析允许研究与治疗相关的癌细胞的转移行为的变化。总的来说,这项研究为胰腺癌的非侵入性治疗提供了一个潜在有效的联合策略.
