胶原蛋白是组织基质的主要成分,大豆可以调节组织免疫反应。两种材料均已用于制造用于组织修复的生物材料。在这项研究中,成人和胎儿人星形胶质细胞在大豆分离蛋白(SPI)-胶原蛋白混合凝胶中或在交联的SPI-胶原蛋白膜的表面上生长。与仅由胶原产生的材料相比,混合材料降低了细胞增殖速率。然而,与对照胶原蛋白材料相比,混合材料没有显着改变细胞运动。RNA测序(RNA-Seq)分析显示细胞周期途径中下调的基因,包括CCNA2,CCNB1,CCNB2,CCND1,CCND2和CDK1,这可能解释了杂化材料中细胞增殖较低。这项研究还揭示了编码细胞外基质(ECM)成分的基因的下调,包括HSPG2,LUM,SDC2,COL4A1,COL4A5,COL4A6和FN1,以及编码趋化因子的基因,包括CCL2、CXCL1、CXCL2、CX3CL1、CXCL3和LIF,与在对照胶原蛋白膜上生长的成年人星形胶质细胞相比,在杂化膜上生长的成年人星形胶质细胞。该研究探索了人类星形胶质细胞对混合材料的细胞和转录反应,并表明该材料在神经修复应用中的潜在有益功能。
Collagen is a major component of the tissue matrix, and soybean can regulate the tissue immune response. Both materials have been used to fabricate biomaterials for tissue repair. In this study, adult and fetal human astrocytes were grown in a soy protein isolate (SPI)-collagen hybrid gel or on the surface of a cross-linked SPI-collagen membrane. Hybrid materials reduced the cell proliferation rate compared to materials generated by collagen alone. However, the hybrid materials did not significantly change the cell motility compared to the control collagen material. RNA-sequencing (RNA-Seq) analysis showed downregulated genes in the cell cycle pathway, including CCNA2, CCNB1, CCNB2, CCND1, CCND2, and CDK1, which may explain lower cell proliferation in the hybrid material. This study also revealed the downregulation of genes encoding extracellular matrix (ECM) components, including HSPG2, LUM, SDC2, COL4A1, COL4A5, COL4A6, and FN1, as well as genes encoding chemokines, including CCL2, CXCL1, CXCL2, CX3CL1, CXCL3, and LIF, for adult human astrocytes grown on the hybrid membrane compared with those grown on the control collagen membrane. The study explored the cellular and transcriptional responses of human astrocytes to the hybrid material and indicated a potential beneficial function of the material in the application of neural repair.