Abstract：
OBJECTIVE: Cold physical plasma (CPP) has emerged as an effective therapy in oncology by inducing cytotoxic effects in various cancer cells, including chondrosarcoma (CS), Ewing\'s sarcoma (ES), and osteosarcoma (OS). The current study investigated the impact of CPP on cell motility in CS (CAL-78), ES (A673), and OS (U2-OS) cell lines, focusing on the actin cytoskeleton.
METHODS: The CASY Cell Counter and Analyzer was used to study cell proliferation and determine the optimal concentrations of fetal calf serum to maintain viability without stimulation of cell proliferation. CellTiter-BlueCell viability assay was used to determine the effects of CPP on the viability of bone sarcoma cells. The Radius assay was used to determine cell migration. Staining for Deoxyribonuclease I, G-actin, and F-actin was used to assay for the effects on the cytoskeleton.
RESULTS: Reductions in cell viability and motility were observed across all cell lines following CPP treatment. CPP induced changes in the actin cytoskeleton, leading to decreased cell motility.
CONCLUSIONS: CPP effectively reduces the motility of bone sarcoma cells by altering the actin cytoskeleton. These findings underscore CPP\'s potential as a therapeutic tool for bone sarcomas and highlight the need for further research in this area.
METHODS: The CASY Cell Counter and Analyzer was used to study cell proliferation and determine the optimal concentrations of fetal calf serum to maintain viability without stimulation of cell proliferation. CellTiter-BlueCell viability assay was used to determine the effects of CPP on the viability of bone sarcoma cells. The Radius assay was used to determine cell migration. Staining for Deoxyribonuclease I, G-actin, and F-actin was used to assay for the effects on the cytoskeleton.
RESULTS: Reductions in cell viability and motility were observed across all cell lines following CPP treatment. CPP induced changes in the actin cytoskeleton, leading to decreased cell motility.
CONCLUSIONS: CPP effectively reduces the motility of bone sarcoma cells by altering the actin cytoskeleton. These findings underscore CPP\'s potential as a therapeutic tool for bone sarcomas and highlight the need for further research in this area.
摘要：
目的：冷物理血浆（CPP）通过在各种癌细胞中诱导细胞毒性作用，已成为肿瘤学中的有效疗法，包括软骨肉瘤（CS），尤因肉瘤(ES),骨肉瘤（OS）。当前的研究调查了CPP对CS（CAL-78）中细胞运动的影响，ES(A673),和OS(U2-OS)细胞系，专注于肌动蛋白细胞骨架。
方法：使用CASY细胞计数器和分析仪研究细胞增殖，并确定胎牛血清的最佳浓度，以维持活力而不刺激细胞增殖。CellTiter-BlueCell活力测定用于确定CPP对骨肉瘤细胞活力的影响。使用Radius测定来确定细胞迁移。脱氧核糖核酸酶Ⅰ染色,G-肌动蛋白,和F-肌动蛋白用于测定对细胞骨架的影响。
结果:在CPP处理后,在所有细胞系中观察到细胞活力和运动性的降低。CPP诱导肌动蛋白细胞骨架的变化，导致细胞运动性下降。
结论：CPP通过改变肌动蛋白细胞骨架有效降低骨肉瘤细胞的运动性。这些发现强调了CPP作为骨肉瘤治疗工具的潜力，并强调了该领域进一步研究的必要性。
方法：使用CASY细胞计数器和分析仪研究细胞增殖，并确定胎牛血清的最佳浓度，以维持活力而不刺激细胞增殖。CellTiter-BlueCell活力测定用于确定CPP对骨肉瘤细胞活力的影响。使用Radius测定来确定细胞迁移。脱氧核糖核酸酶Ⅰ染色,G-肌动蛋白,和F-肌动蛋白用于测定对细胞骨架的影响。
结果:在CPP处理后,在所有细胞系中观察到细胞活力和运动性的降低。CPP诱导肌动蛋白细胞骨架的变化，导致细胞运动性下降。
结论：CPP通过改变肌动蛋白细胞骨架有效降低骨肉瘤细胞的运动性。这些发现强调了CPP作为骨肉瘤治疗工具的潜力，并强调了该领域进一步研究的必要性。
