该研究检查了纯乙酰-11-酮-β-乳香酸(AKBA)的抗菌和抗氧化潜力,乳香酸(70%)和AKBA负载的纳米颗粒作为局部聚合物膜。纯AKBA的优化浓度(0.05%w/v),乳香酸(BA),和AKBA负载的银纳米粒子用于研究其对薄膜特性的影响。羧甲基纤维素(CMC),海藻酸钠(SA),本研究制备了明胶(Ge)复合薄膜。评估聚合物膜的生物学(抗氧化和抗微生物活性)和机械特性,例如拉伸强度(TS)和伸长率(%)。此外,还评估了其他参数,包括膜的防水性能和颜色属性。此外,使用FTIR等分析技术进行评估,XRD,和SEM。表面分析显示AgNP沉淀导致膜结构中的一些颗粒。总的来说,结果表明微观结构相对一致。此外,由于AKBA的加入,BA,和AgNPs,TS显著下降,水分含量,水溶性,并观察到水蒸气的渗透。薄膜的透明度也呈下降趋势,并且颜色分析显示膜的黄度(b*)降低。重要的是,在CSG膜中观察到对DPPH自由基和ABTS阳离子的抗氧化活性显着增加。此外,负载AgNP-AKBA的膜对白色念珠菌显示出显著的抗真菌活性。此外,分子对接分析揭示了AKBA之间的分子间相互作用,AgNPs,和复合膜。对接结果表明AKBA和银纳米颗粒与明胶和羧甲基纤维素分子的良好结合。总之,这些聚合物膜具有作为具有显著抗氧化和抗真菌活性的新型材料的潜力。
The study examined the antimicrobial and antioxidant potential of pure Acetyl-11-keto-β-boswellic acid (AKBA), boswellic acid (70%) and AKBA loaded nanoparticles as topical polymeric films. The optimized concentration (0.05 % w/v) of pure AKBA, boswellic acid (BA), and AKBA loaded silver nanoparticles were used to study its impact on film characteristics. Carboxymethyl cellulose (CMC), sodium alginate (SA), and gelatin (Ge) composite films were prepared in this study. The polymeric films were evaluated for their biological (antioxidant and antimicrobial activities) and mechanical characteristics such as tensile strength (TS) and elongation (%). Moreover, other parameters including water barrier properties and color attributes of the film were also evaluated. Furthermore, assessments were conducted using analytical techniques like FTIR, XRD, and SEM. Surface analysis revealed that AgNP precipitation led to a few particles in the film structure. Overall, the results indicate a relatively consistent microstructure. Moreover, due to the addition of AKBA, BA, and AgNPs, a significant decrease in TS, moisture content, water solubility, and water vapor permeation was observed. The films transparency also showed a decreasing trend, and the color analysis revealed decreasing yellowness (b*) of the films. Importantly, a significant increase in antioxidant activity against DPPH free radicals and ABTS cations was observed in the CSG films. Additionally, the AgNP-AKBA loaded films displayed significant antifungal activity against C. albicans. Moreover, the molecular docking analysis revealed the inter-molecular interactions between the AKBA, AgNPs, and composite films. The docking results indicate good binding of AKBA and silver nanoparticles with gelatin and carboxymethyl cellulosemolecules. In conclusion, these polymeric films have potential as novel materials with significant antioxidant and antifungal activities.