PDF(Pubmed)
Abstract：
Prolonged pressure on the skin can result in pressure ulcers, which may lead to serious complications, such as infection and tissue damage. In this study, we evaluated the effect of a carboxymethyl cellulose/gelatin/sodium alginate (CMC/Gel/Alg) hydrogel containing N-acetyl-cysteine (NAC) on the healing of pressure ulcers. Pressure ulcers were induced by applying a magnet to the dorsum of rat skin. The wounds were then treated with sterile gauze, ChitoHeal Gel®, and CMC/Gel/Alg hydrogel dressings with or without NAC for the other groups. We evaluated the morphology, weight loss, swelling, rheology, blood compatibility, cytocompatibility, antioxidant capacity, and wound scratch of the prepared hydrogel. MTT assay revealed that the optimum concentration of NAC was 5 mg/ml, which induced higher cell proliferation and viability. Results of the histopathological evaluation showed increased wound closure, and complete re-epithelialization in the hydrogel-containing NAC group compared to the other groups. The CMC/Gel/Alg/5 mg/ml NAC hydrogel dressing showed 84% wound closure at 14 days after treatment. Immunohistochemical results showed a decrease in the level of TNF-α on day 14 compared day 7. Results of the qPCR assay revealed that NAC hydrogel increased the expression of Collagen type I and TGF-β1 and decreased MMP2 and MMP9 mRNA on the 14th day. The results suggest that the CMC/Gel/Alg/5 mg/ml NAC hydrogel with antioxidant properties is an appropriate dressing for wound healing.
摘要：
长时间压迫皮肤会导致压疮，这可能会导致严重的并发症，如感染和组织损伤。在这项研究中,我们评估了含有N-乙酰半胱氨酸（NAC）的羧甲基纤维素/明胶/海藻酸钠（CMC/Gel/Alg）水凝胶对压疮愈合的影响。通过将磁铁施加到大鼠皮肤的背部来诱发压力性溃疡。然后用无菌纱布处理伤口，ChitoHeal凝胶®,和CMC/Gel/Alg水凝胶敷料,对于其他组具有或不具有NAC。我们评估了形态学，减肥,肿胀,流变学,血液相容性,细胞相容性,抗氧化能力，和所制备的水凝胶的伤口划痕。MTT分析表明，NAC的最佳浓度为5mg/ml，诱导更高的细胞增殖和活力。组织病理学评估结果显示伤口闭合增加，与其他组相比，含有水凝胶的NAC组的上皮再形成完全。CMC/Gel/Alg/5mg/mlNAC水凝胶敷料在治疗后14天显示84%的伤口闭合。免疫组织化学结果表明，与第7天相比，第14天的TNF-α水平降低。qPCR检测结果表明，在第14天，NAC水凝胶增加了I型胶原蛋白和TGF-β1的表达，并降低了MMP2和MMP9mRNA的表达。结果表明，具有抗氧化性能的CMC/Gel/Alg/5mg/mlNAC水凝胶是伤口愈合的合适敷料。
