利什曼病,欧洲最重要的人畜共患病之一,是由婴儿利什曼原虫引起的,细胞内原生动物寄生虫。这种疾病在地中海地区很流行,主要水库是狗。一些研究表明,在某些犬科动物中,可能具有对婴儿乳球菌感染的易感性。其中之一是拳击手品种,这表明疾病的患病率很高。在这项研究中,评估了居住在地中海地区的拳击手犬血清样品的免疫学和基因组特征,以分析免疫反应和这种易感性的可能遗传解释。血清细胞因子IFN-γ,IL-2,IL-6,IL-8和IL-18通过ELISA商业测试测定,而基因分型研究是使用CanineHDDNA分析BeadChip进行的。结果表明,与其他犬科动物品种的公开数据相比,血清细胞因子水平存在相关差异,以及序列变化可以解释拳击手品种对疾病的高度易感性。具体而言,CIITA中的多态性变异,HSF2BP,LTBP1,MITF,NOXA1,PKIB,RAB38RASEF,发现TLE1和TLR4基因,这可以解释该品种对婴儿乳球菌感染的易感性。
Leishmaniosis, one of the most important zoonoses in Europe, is caused by Leishmania infantum, an intracellular protozoan parasite. This disease is endemic in the Mediterranean area, where the main reservoir is the dog. Several studies indicate a possible susceptibility to L. infantum infection with clinical signs in some canine breeds. One of them is the boxer breed, which shows a high prevalence of disease. In this study, immunological and genomic characterization of serum samples from boxer dogs living in the Mediterranean area were evaluated to analyze the immune response and the possible genetic explanation for this susceptibility. Serum levels of cytokines IFN-γ, IL-2, IL-6, IL-8, and IL-18 were determined by ELISA commercial tests, while the genotyping study was performed using the CanineHD DNA Analysis BeadChip. The results show relevant differences in the serum levels of cytokines compared to published data on other canine breeds, as well as sequence changes that could explain the high susceptibility of the boxer breed to the disease. Concretely, polymorphic variants in the CIITA, HSF2BP, LTBP1, MITF, NOXA1, PKIB, RAB38, RASEF, TLE1, and TLR4 genes were found, which could explain the susceptibility of this breed to L. infantum infection.