藻红蛋白和多糖在医药上具有显著的商业价值,化妆品,和食品工业由于其优异的生物活性功能。为了最大限度地生产生物质,藻红蛋白,和紫菜中的多糖,培养基补充葡萄糖酸钙(CG),葡萄糖酸镁(MG)和多肽(BT),并在三个单因素实验的基础上,使用响应面法(RSM)确定了它们的最佳用量。CG的最佳浓度,MG,和BT分别测定为4、12和2gL-1。基于RSM的模型表明,生物量和藻红蛋白的产生仅受到MG和BT的显着影响。分别。然而,多糖生产受到CG和BT之间以及MG和BT之间相互作用的显着影响,单独使用BT没有显著影响。利用优化的培养条件,最大生物量(5.97gL-1),藻红蛋白(102.95mgL-1),和多糖(1.42gL-1)浓度达到甚至超过模型预测的最大值。优化后,生物量,藻红蛋白,多糖浓度增加了132.3%,27.97%,和136.67%,分别,与对照相比。总的来说,本研究为利用紫癜高效生产藻红蛋白和多糖奠定了坚实的基础。
Phycoerythrin and polysaccharides have significant commercial value in medicine, cosmetics, and food industries due to their excellent bioactive functions. To maximize the production of biomass, phycoerythrin, and polysaccharides in Porphyridium purpureum, culture media were supplemented with calcium gluconate (CG), magnesium gluconate (MG) and polypeptides (BT), and their optimal amounts were determined using the response surface methodology (RSM) based on three single-factor experiments. The optimal concentrations of CG, MG, and BT were determined to be 4, 12, and 2 g L-1, respectively. The RSM-based models indicated that biomass and phycoerythrin production were significantly affected only by MG and BT, respectively. However, polysaccharide production was significantly affected by the interactions between CG and BT and those between MG and BT, with no significant effect from BT alone. Using the optimized culture conditions, the maximum biomass (5.97 g L-1), phycoerythrin (102.95 mg L-1), and polysaccharide (1.42 g L-1) concentrations met and even surpassed the model-predicted maximums. After optimization, biomass, phycoerythrin, and polysaccharides concentrations increased by 132.3%, 27.97%, and 136.67%, respectively, compared to the control. Overall, this study establishes a strong foundation for the highly efficient production of phycoerythrin and polysaccharides using P. purpureum.