■昆虫生物胺在调节行为和生理过程中起重要作用。它们通过与生物胺受体(BARs)结合而发挥作用,它们是G蛋白偶联受体超家族中的特异性受体蛋白。BAR基因已从多种模式昆虫中克隆和表征,包括黑腹果蝇,冈比亚按蚊,家蚕,蜜蜂和栗树。然而,解决分子性质的工作相对较少,表达式配置文件,和来自其他昆虫的BARs的药理学表征,包括重要的害虫。
结果:在这项研究中,我们从小菜蛾中克隆了17个编码推定的生物胺受体蛋白的基因,芸苔属作物的一种全球性害虫。这些PxBAR基因是五个章鱼胺受体(PxOA1,PxOA2B1,PxOA2B2,PxOA2B3和PxOA3),三种酪胺受体(PxTAR1A,PxTAR1B,和PxTAR2),四种多巴胺受体(PxDOP1,PxDOP2,PxDOP3和PxDopEcR),和五种5-羟色胺受体(Px5-HT1A,Px5-HT1B,Px5-HT2A,Px5-HT2B,和Px5-HT7)。所有PxBAR均与直系同源BAR显示出相当大的序列同一性,和系统发育分析将受体聚集在各自的群体中,同时保留了有机体的进化关系。我们研究了它们的分子特性和表达谱,药理学上表征多巴胺受体,PxDOP2.
结论:我们的研究提供了有关木鸟的生物胺受体的重要信息和资源。这表明了控制这种害虫物种的潜在目标地点。©2021年化学工业学会。
UNASSIGNED: Insect biogenic amines play important roles in mediating behavioral and physiological processes. They exert their effects by binding to biogenic amine receptors (BARs), which are specific receptor proteins in the G-protein-coupled receptor superfamily. BAR genes have been cloned and characterized from multiple model insects, including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera and Tribolium castaneum. However, relatively little work has addressed the molecular properties, expression profiles, and pharmacological characterization of BARs from other insects, including important pests.
RESULTS: In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5-HT1A , Px5-HT1B , Px5-HT2A , Px5-HT2B , and Px5-HT7 ). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2.
CONCLUSIONS: Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species. © 2021 Society of Chemical Industry.