RESULTS: In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5-HT1A , Px5-HT1B , Px5-HT2A , Px5-HT2B , and Px5-HT7 ). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2.
CONCLUSIONS: Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species. © 2021 Society of Chemical Industry.
结果:在这项研究中,我们从小菜蛾中克隆了17个编码推定的生物胺受体蛋白的基因,芸苔属作物的一种全球性害虫。这些PxBAR基因是五个章鱼胺受体(PxOA1,PxOA2B1,PxOA2B2,PxOA2B3和PxOA3),三种酪胺受体(PxTAR1A,PxTAR1B,和PxTAR2),四种多巴胺受体(PxDOP1,PxDOP2,PxDOP3和PxDopEcR),和五种5-羟色胺受体(Px5-HT1A,Px5-HT1B,Px5-HT2A,Px5-HT2B,和Px5-HT7)。所有PxBAR均与直系同源BAR显示出相当大的序列同一性,和系统发育分析将受体聚集在各自的群体中,同时保留了有机体的进化关系。我们研究了它们的分子特性和表达谱,药理学上表征多巴胺受体,PxDOP2.
结论:我们的研究提供了有关木鸟的生物胺受体的重要信息和资源。这表明了控制这种害虫物种的潜在目标地点。©2021年化学工业学会。