布鲁氏菌病是由兼性细胞内布鲁氏菌病原体引起的应报告疾病。在这项研究中,对来自埃及的8株流产布鲁氏菌和18株布鲁氏菌进行了注释,并分别与RB51和REV1疫苗进行了比较。BV-BRC服务器中的RAST工具包用于注释,揭示基因组长度为3,250,377bp和3,285,803bp,3289和3323CDS,48和49tRNA基因,相同数量的rRNA(3)基因,583和586个假设的蛋白质,分别为流产芽孢杆菌和melitensis的2697和2726个功能蛋白。流产芽孢杆菌菌株表现出相似数量的候选基因,而B.melitensis菌株表现出一些差异,特别是在SRR19520422Faiyum菌株中。此外,B.melitensis澄清了抗菌素抗性基因的差异(KatG,FabL,MTRA,MtrB,OxyR,和VanO型)在SRR19520319Faiyum和(ErmC和TetK)在SRR19520422Faiyum菌株中。此外,全基因组系统发育分析证明,所有流产芽孢杆菌菌株都与接种动物有关,所有梅诺非亚芽孢杆菌菌株都聚集在一起,与Gharbia密切相关,Dameitta,还有KafrElshiek.Bowtie2工具沿着基因组鉴定出338(8个流产B.abortus)和4271(18个melitensis)单核苷酸多态性(SNP)。这些变体已根据类型和影响进行了注释。此外,预测了30个候选基因,并将其提交给GenBank(B.abortus中的24个)和(B.melitensis中的6个)。这项研究为遗传变异提供了重要的见解,毒力因子,和布鲁氏菌病原体的疫苗相关关联,加强我们对埃及布鲁氏菌病流行病学和进化的了解。
Brucellosis is a notifiable disease induced by a facultative intracellular Brucella pathogen. In this study, eight Brucella abortus and eighteen Brucella melitensis strains from Egypt were annotated and compared with RB51 and REV1 vaccines respectively. RAST toolkit in the BV-BRC server was used for annotation, revealing genome length of 3,250,377 bp and 3,285,803 bp, 3289 and 3323 CDS, 48 and 49 tRNA genes, the same number of rRNA (3) genes, 583 and 586 hypothetical proteins, 2697 and 2726 functional proteins for B. abortus and B. melitensis respectively. B. abortus strains exhibit a similar number of candidate genes, while B. melitensis strains showed some differences, especially in the SRR19520422 Faiyum strain. Also, B. melitensis clarified differences in antimicrobial resistance genes (KatG, FabL, MtrA, MtrB, OxyR, and VanO-type) in SRR19520319 Faiyum and (Erm C and Tet K) in SRR19520422 Faiyum strain. Additionally, the whole genome phylogeny analysis proved that all B. abortus strains were related to vaccinated animals and all B. melitensis strains of Menoufia clustered together and closely related to Gharbia, Dameitta, and Kafr Elshiek. The Bowtie2 tool identified 338 (eight B. abortus) and 4271 (eighteen B. melitensis) single nucleotide polymorphisms (SNPs) along the genomes. These variants had been annotated according to type and impact. Moreover, thirty candidate genes were predicted and submitted at GenBank (24 in B. abortus) and (6 in B. melitensis). This study contributes significant insights into genetic variation, virulence factors, and vaccine-related associations of Brucella pathogens, enhancing our knowledge of brucellosis epidemiology and evolution in Egypt.