啤酒废粮(BSG)是啤酒行业的主要副产品。BSG富含多种营养成分,以及对其有效的搜索,高价值利用正在进行中。用BSG底物发酵枯草芽孢杆菌生产环境益生菌因子γ-PGA,并对发酵籽粒成分进行分析。γ-PGA产量达到31.58±0.21g/kgBSG。气相色谱-质谱和非靶向代谢组学分析揭示了发酵谷物中的73种新的挥发性物质。此外,2,376种代谢物在发酵后被上调,几种成分对植物生长和发育有益(如外胎,乙酰丁香酚,L-苯丙氨酸,烟酸,异戊二烯,泛酸,多巴胺,甘氨酸,脯氨酸,茉莉酸,etc).这些结果表明可以合成足够量的γ-PGA用作功能性肥料。
Brewer\'s spent grain (BSG) is a main byproduct of the beer industry. BSG is rich in a variety of nutrients, and the search for its effective, high-value utilization is ongoing. Environmental probiotic factor γ-PGA was produced by fermenting Bacillus subtilis with BSG substrate and the fermenting grain components were analyzed. The γ-PGA yield reached 31.58 ± 0.21 g/kg of BSG. Gas chromatography-mass spectrometry and non-targeted metabolomics analyses revealed 73 new volatile substances in the fermenting grains. Furthermore, 2,376 metabolites were upregulated after fermentation and several components were beneficial for plant growth and development (such as ectoine, acetyl eugenol, L-phenylalanine, niacin, isoprene, pantothenic acid, dopamine, glycine, proline, jasmonic acid, etc). These results show that it is possible to synthesize adequate amounts of γ-PGA for use as a functional fertilizer.