含有大量饱和脂肪酸和简单碳水化合物的高度加工食品的消费增加是超重和肥胖负担的主要原因。此外,众所周知,不健康的饮食与慢性压力暴露相结合,与中枢神经系统疾病的患病率增加有关。在本研究中,应用全脑蛋白质组方法探索暴露于西方饮食和/或应激后的蛋白质变化.用西方饮食和人类零食喂养雌性成年大鼠和/或经受由社会不稳定引起的慢性应激12周。西方饮食的消费导致肥胖表型并诱导血清代谢参数的变化。食用西方饮食仅导致5.4%的蛋白质发生变化,而所有检测到的蛋白质中有48%受到慢性压力的影响,其中86.3%由于暴露于慢性压力而下调。然而,用特定的饮食喂养可以改善应激诱导的大脑蛋白质组变化。参与轴突发生和介导AMPA谷氨酸受体(Nptx1)突触聚集的蛋白质的下调,以及与代谢过程相关的蛋白质(Atp5i,Mrps36,Ndufb4),被确认,尽管检测到参与CNS发育和分化的蛋白质(Basp1,Cend1)的表达增加,对压力的反应,学习和记忆(Prrt2),和调节突触传递(Ncam1,Prrt2)。总之,全球蛋白质组分析提供了有关西方饮食和压力暴露组合对脑皮质蛋白改变的影响的信息,并深入了解了文献中描述的大脑功能和形态改变以及行为障碍的潜在机制和途径。
The increasing consumption of highly processed foods with high amounts of saturated fatty acids and simple carbohydrates is a major contributor to the burden of overweight and obesity. Additionally, an unhealthy diet in combination with chronic stress exposure is known to be associated with the increased prevalence of central nervous system diseases. In the present study, the global brain proteome approach was applied to explore protein alterations after exposure to the Western diet and/or stress. Female adult rats were fed with the Western diet with human snacks and/or subjected to chronic stress induced by social instability for 12 weeks. The consumption of the Western diet resulted in an obese phenotype and induced changes in the serum metabolic parameters. Consuming the Western diet resulted in changes in only 5.4% of the proteins, whereas 48% of all detected proteins were affected by chronic stress, of which 86.3% were down-regulated due to this exposure to chronic stress. However, feeding with a particular diet modified stress-induced changes in the brain proteome. The down-regulation of proteins involved in axonogenesis and mediating the synaptic clustering of AMPA glutamate receptors (Nptx1), as well as proteins related to metabolic processes (Atp5i, Mrps36, Ndufb4), were identified, while increased expression was detected for proteins involved in the development and differentiation of the CNS (Basp1, Cend1), response to stress, learning and memory (Prrt2), and modulation of synaptic transmission (Ncam1, Prrt2). In summary, global proteome analysis provides information about the impact of the combination of the Western diet and stress exposure on cerebrocortical protein alterations and yields insight into the underlying mechanisms and pathways involved in functional and morphological brain alterations as well as behavioral disturbances described in the literature.