背景:食糖行为对蚊子的生存和繁殖至关重要,并被用来开发新的控制策略,例如有吸引力的目标糖饵(ATSB)。这项研究检查了登革热媒介的食糖习惯,埃及伊蚊,在半野外条件下,以确定糖食的最佳时间(年龄),以及糖源的可用性是否会影响这些蚊子的血液喂养。
方法:进行了一系列配对选择测定,其中允许蚊子在直接来自兔子的糖粉或血粉之间进行选择。雌性1天大的蚊子在笼子I-V中被给予膳食选择,并且从笼子I至V开始,每天仅在一个笼子中观察喂养选择,持续5天。Ae的偏好。在不同的时间和生理年龄评估了埃及伊蚊以糖或血液为食,以及糖源可用性对血液喂养的影响。
结果:在出现后的前5天,蚊子对糖或血餐的偏好没有显着差异。然而,在第一个促性腺激素周期之后,他们更喜欢血液而不是糖(赔率比,或[95%置信区间,CI]=9.4[6.7-13.0];P<0.001)。未产Ae.如果糖和血液来源同时可用,则埃及伊蚊(≤5天大的蚊子)不太可能采血(OR=0.06[0.02-0.16];P<0.001)。
结论:新出现的女性Ae。埃及伊蚊同样可能选择糖餐或血餐。然而,在第一个促性腺激素周期之后,他们更喜欢血液而不是糖。此外,当糖和血液来源都可用时,未产雌性蚊子不太可能采血。这些发现为Ae的糖摄食行为提供了见解。埃及伊蚊,可以为开发和优化新的控制策略提供信息,例如使用ATSB。
BACKGROUND: Sugar-feeding behaviour is essential for mosquito survival and reproduction, and has been exploited to develop new control strategies, such as the attractive targeted sugar baits (ATSB). This study examined the sugar-feeding habits of the dengue vector, Aedes aegypti, in semi-field conditions to determine the optimal timing (age) of sugar meals and whether the availability of sugar sources could affect blood-feeding by these mosquitoes.
METHODS: A series of paired-choice assays were conducted in which mosquitoes were allowed to choose between a sugar meal or a blood meal directly from a rabbit. Female 1-day-old mosquitoes were given meal choices in cages I-V and observed for feeding choice in only one cage every day for 5 days starting with cages I to V. The preference of Ae. aegypti to feed on sugar or blood and the effect of sugar source availability on blood-feeding was assessed at different chronological and physiological ages.
RESULTS: In the first 5 days post-emergence, there was no significant difference in mosquito preference for sugar or blood meals. However, after the first gonotrophic cycle, they had a greater preference for blood over sugar (odds ratio, OR [95% confidence interval, CI] = 9.4 [6.7-13.0]; P < 0.001). Nulliparous Ae. aegypti females (≤ 5-day-old mosquitoes) were less likely to blood-feed if both sugar and blood sources were concurrently available (OR = 0.06 [0.02-0.16]; P < 0.001).
CONCLUSIONS: Newly emerged females of Ae. aegypti mosquitoes were equally likely to choose a sugar meal or a blood meal. However, after the first gonotrophic cycle, they had a greater preference for blood over sugar. Additionally, nulliparous female mosquitoes were less likely to blood-feed when both sugar and blood sources were available. These findings provide insights into the sugar-feeding behaviour of Ae. aegypti and can inform the development and optimization of new control strategies such as using ATSB.