PDF(Pubmed)
Abstract：
Mosquitoes such as Aedes aegypti must consume a blood meal for the nutrients necessary for egg production. Several transcriptome and proteome changes occur post blood meal that likely corresponds with codon usage alterations. Transfer RNA (tRNA) is the adapter molecule that reads messenger RNA (mRNA) codons to add the appropriate amino acid during protein synthesis. Chemical modifications to tRNA enhance codons\' decoding, improving the accuracy and efficiency of protein synthesis. Here, we examined tRNA modifications and transcripts associated with the blood meal and subsequent periods of vitellogenesis in A. aegypti. More specifically, we assessed tRNA transcript abundance and modification levels in the fat body at critical times post blood-feeding. Based on a combination of alternative codon usage and identification of particular modifications, we identified that increased transcription of tyrosine tRNAs is likely critical during the synthesis of egg yolk proteins in the fat body following a blood meal. Altogether, changes in both the abundance and modification of tRNA are essential factors in the process of vitellogenin production after blood-feeding in mosquitoes.
摘要：
埃及伊蚊等蚊子必须消耗血粉来获取产蛋所需的营养。血餐后发生几种转录组和蛋白质组变化，可能与密码子使用改变相对应。转移RNA(tRNA)是在蛋白质合成期间读取信使RNA(mRNA)密码子以添加适当氨基酸的衔接子分子。对tRNA的化学修饰增强密码子解码，提高蛋白质合成的准确性和效率。这里,我们检查了与血粉和随后的卵黄发生时期相关的tRNA修饰和转录本。更具体地说,我们在采血后的关键时刻评估了脂肪体内的tRNA转录物丰度和修饰水平.基于替代密码子使用和特定修饰的识别的组合，我们发现，酪氨酸tRNA转录的增加可能在血餐后脂肪体内蛋黄蛋白合成过程中至关重要。总之,tRNA丰度和修饰的变化是蚊子采血后卵黄蛋白原生成过程中的重要因素。
