背景:鲍曼不动杆菌(A.鲍曼不动杆菌)是一种机会致病菌,主要与医院获得性感染和长期住院的免疫功能低下个体有关。近年来,它对许多不同类型的抗生素越来越有抵抗力。金属-β-内酰胺酶(MBL)的产生是这种抗性的主要原因之一。本研究旨在检测烧伤患者鲍曼不动杆菌分离株中属于维罗纳整合素金属β-内酰胺酶(bla-VIM)和亚胺酶(bla-IMP)组的MBL基因的存在。
方法:使用传统方法,然后使用VITEK2(BioMérieux,LesPennes-Mirabeau,法国)识别系统。通过在Muller-Hinton琼脂上使用亚胺培南圆盘在亚胺培南抗性菌株中检测到金属β-内酰胺酶。聚合酶链反应(PCR)技术用于检查117个分离株,以检测编码基因的MBL,例如bla-VIM,还有BLA-IMP.
结果:在78.6%的患者中检测到亚胺培南耐药。分离物的PCR检测鉴定出bla-VIM-1,bla-VIM-2,bla-IMP-1和bla-IMP-2基因的比例为17%,40.1%,29.9%和4.2%,分别。
结论:研究结果表明,大多数鲍曼不动杆菌分离株含有一个或多个检测到的基因,这表明MBL的产生在抗性机制中起着关键作用。
BACKGROUND: Acinetobacter baumannii (A. baumannii) is an opportunistic pathogenic bacterium mainly associated with hospital acquired infections and in immunocompromised individuals who stay in hospitals for a long time. In recent years, it has become increasingly resistant to many different types of antibiotics. The production of the metallo-beta-lactamase (MBL) enzyme is one of the primary causes of this resistance. This study aimed to detect the presence of MBL genes that belong to the verona integrin metallo-β-lactamase (bla-VIM) and imipenemase (bla-IMP) groups in the isolates of Acinetobacter baumannii from burn patients.
METHODS: One hundred and seventeen (117) isolates of A. baumannii were obtained from patient specimens using traditional methods followed by using the VITEK 2 (BioMérieux, Les Pennes-Mirabeau, France) identification system. Metallo β-lactamases were detected in the imipenem-resistant strains by using imipenem disks on Muller-Hinton agar. The polymerase chain reaction (PCR) technique was utilized to examine 117 isolates for the detection of MBLs encoding genes such as bla-VIM, and bla-IMP.
RESULTS: Imipenem resistance was detected in 78.6% of the patients. The PCR assays of the isolates identified bla-VIM-1, bla-VIM-2, bla-IMP-1 and bla-IMP-2 genes at the rates of 17%, 40.1%, 29.9% and 4.2%, respectively.
CONCLUSIONS: The findings suggest that the majority of A. baumannii isolates harbour one or more of the detected genes, signifying that the production of MBLs plays a pivotal role in resistance mechanisms.