METHODS: One hundred and seventeen (117) isolates of A. baumannii were obtained from patient specimens using traditional methods followed by using the VITEK 2 (BioMérieux, Les Pennes-Mirabeau, France) identification system. Metallo β-lactamases were detected in the imipenem-resistant strains by using imipenem disks on Muller-Hinton agar. The polymerase chain reaction (PCR) technique was utilized to examine 117 isolates for the detection of MBLs encoding genes such as bla-VIM, and bla-IMP.
RESULTS: Imipenem resistance was detected in 78.6% of the patients. The PCR assays of the isolates identified bla-VIM-1, bla-VIM-2, bla-IMP-1 and bla-IMP-2 genes at the rates of 17%, 40.1%, 29.9% and 4.2%, respectively.
CONCLUSIONS: The findings suggest that the majority of A. baumannii isolates harbour one or more of the detected genes, signifying that the production of MBLs plays a pivotal role in resistance mechanisms.
方法:使用传统方法,然后使用VITEK2(BioMérieux,LesPennes-Mirabeau,法国)识别系统。通过在Muller-Hinton琼脂上使用亚胺培南圆盘在亚胺培南抗性菌株中检测到金属β-内酰胺酶。聚合酶链反应(PCR)技术用于检查117个分离株,以检测编码基因的MBL,例如bla-VIM,还有BLA-IMP.
结果:在78.6%的患者中检测到亚胺培南耐药。分离物的PCR检测鉴定出bla-VIM-1,bla-VIM-2,bla-IMP-1和bla-IMP-2基因的比例为17%,40.1%,29.9%和4.2%,分别。
结论:研究结果表明,大多数鲍曼不动杆菌分离株含有一个或多个检测到的基因,这表明MBL的产生在抗性机制中起着关键作用。