背景:生物乳化剂是天然或基于微生物的产品,具有在水中乳化疏水性化合物的能力。这些化合物是可生物降解的,环保,并在各种行业中找到应用。
结果:在亚历山大从不同来源分离出13株酵母,埃及,并评估其产生细胞内生物乳化剂的潜力。一种酵母,与埃及当地市场隔绝,乳化指数(EI24)值最高。通过26SrRNA测序,该酵母被鉴定为酿酒酵母菌株MYN04。研究了分离物的生长动力学,孵育36小时后,细胞干重(CDW)产量最高,为3.17g/L,EI24为55.6%。实验设计用于研究培养参数对最大化生物乳化剂SC04产量和CDW的影响。该研究实现了79.0±2.0%的最大EI24。此外,粗生物乳化剂用50%乙醇沉淀,并使用SephadexG-75凝胶过滤色谱法纯化。发现生物乳化剂SC04由27.1%的碳水化合物和72.9%的蛋白质组成。纯化的生物乳化剂SC04的结构测定使用傅里叶变换红外光谱(FTIR),扫描电子显微镜-能量色散X射线能谱(SEM-EDX),高效液相色谱(HPLC),和核磁共振波谱(NMR)。FTIR光谱揭示了与碳水化合物的羧基和羟基相关的特征带,以及蛋白质的胺基。HPLC剖析检测到存在甘露糖的单糖构成,半乳糖,和葡萄糖。凝胶过滤后级分的物理化学表征表明生物乳化剂SC04是高分子量的蛋白质-寡糖复合物。这种生物乳化剂在不同的pH值下表现出稳定性,温度,和盐度。浓度为0.5mg/mL时,它表现出51.8%的DPPH自由基清除。此外,使用MTT测定法进行的体外细胞毒性评估显示,SC04对正常上皮肾细胞系具有非细胞毒性作用。
结论:这项研究提出了一种新的生态友好型生物乳化剂,命名为SC04,具有显著的乳化能力,抗氧化和抗癌特性,和稳定性能。这些发现表明,SC04是一种很有前途的候选食品,Pharmaceutical,和工业部门。
BACKGROUND: Bioemulsifiers are natural or microbial-based products with the ability to emulsify hydrophobic compounds in water. These compounds are biodegradable, eco-friendly, and find applications in various industries.
RESULTS: Thirteen yeasts were isolated from different sources in Alexandria, Egypt, and evaluated for their potential to produce intracellular bioemulsifiers. One yeast, isolated from a local market in Egypt, showed the highest emulsification index (EI24) value. Through 26S rRNA sequencing, this yeast was identified as Saccharomyces cerevisiae strain MYN04. The growth kinetics of the isolate were studied, and after 36 h of incubation, the highest yield of cell dry weight (CDW) was obtained at 3.17 g/L, with an EI24 of 55.6%. Experimental designs were used to investigate the effects of culture parameters on maximizing bioemulsifier SC04 production and CDW. The study achieved a maximum EI24 of 79.0 ± 2.0%. Furthermore, the crude
bioemulsifier was precipitated with 50% ethanol and purified using Sephadex G-75 gel filtration chromatography.
Bioemulsifier SC04 was found to consist of 27.1% carbohydrates and 72.9% proteins. Structural determination of purified
bioemulsifier SC04 was carried out using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), high-performance liquid chromatography (HPLC), and nuclear magnetic resonance spectroscopy (NMR). FTIR spectroscopy revealed characteristic bands associated with carboxyl and hydroxyl groups of carbohydrates, as well as amine groups of proteins. HPLC analysis of monosaccharide composition detected the presence of mannose, galactose, and glucose. Physicochemical characterization of the fraction after gel filtration indicated that
bioemulsifier SC04 is a high molecular weight protein-oligosaccharide complex. This
bioemulsifier demonstrated stability at different pH values, temperatures, and salinities. At a concentration of 0.5 mg/mL, it exhibited 51.8% scavenging of DPPH radicals. Furthermore, in vitro cytotoxicity evaluation using the MTT assay revealed a noncytotoxic effect of SC04 against normal epithelial kidney cell lines.
CONCLUSIONS: This study presents a new eco-friendly bioemulsifier, named SC04, which exhibits significant emulsifying ability, antioxidant and anticancer properties, and stabilizing properties. These findings suggest that SC04 is a promising candidate for applications in the food, pharmaceutical, and industrial sectors.