{Reference Type}: Journal Article {Title}: An Insightful Overview of Microbial Biosurfactant: A Promising Next-Generation Biomolecule for Sustainable Future. {Author}: Thundiparambil Venu A;Vijayan J;Ammanamveetil MHA;Kottekkattu Padinchati K; {Journal}: J Basic Microbiol {Volume}: 0 {Issue}: 0 {Year}: 2024 Jun 27 {Factor}: 2.65 {DOI}: 10.1002/jobm.202300757 {Abstract}: Microbial biosurfactant is an emerging vital biomolecule of the 21st century. They are amphiphilic compounds produced by microorganisms and possess unique properties to reduce surface tension activity. The use of microbial surfactants spans most of the industrial fields due to their biodegradability, less toxicity, being environmentally safe, and being synthesized from renewable sources. These would be highly efficient eco-friendly alternatives to petroleum-derived surfactants that would open up new approaches to research on the production of biosurfactants. In the upcoming era, biobased surfactants will become a dominating multifunctional compound in the world market. Research on biosurfactants ranges from the search for novel microorganisms that can produce new molecules, structural and physiochemical characterization of biosurfactants, and fermentation process for enhanced large-scale productivity and green applications. The main goal of this review is to provide an overview of the recent state of knowledge and trends about microbially derived surfactants, various aspects of biosurfactant production, definition, properties, characteristics, diverse advances, and applications. This would lead a long way in the production of biosurfactants as globally successful biomolecules of the current century.