角膜损伤在全球视力障碍中起着重要作用,强调对具有附着力等特定属性的创新生物材料的需求,凝聚力,和再生潜力。在这项研究中,我们开发了一种用于角膜重建的生物相容性生物粘合剂.来源于I型胶原蛋白,天然存在于人类角膜基质组织中,生物粘合剂与改性聚乙二醇二丙烯酸酯(PEGDA-DOPA)交联,使其可固化通过可见光暴露和表现出优异的粘附生物组织,即使在潮湿的条件下。通过操纵其前体聚合物的浓度并调节光交联的持续时间来定制所提出的生物粘合剂的物理化学特性。为了确定具有最大附着力的最佳样品,机械强度,和生物相容性,进行了表征测试。最佳试样,由30%(w/v)PEGDA-DOPA组成,用可见光固化5分钟,表现出良好的粘合强度783.6kPa和剪切强度53.7kPa,超过了商业化的眼部粘合剂。此外,生物相容性测试结果表明,在培养7天后,接种在水凝胶粘合剂上的角膜细胞的存活率显著高(>100%)。因此,这种设计的生物粘合剂,具有高粘合强度,强大的机械强度,和优异的生物相容性,有望增强受损角膜基质组织的自发修复过程。
Corneal injuries play a significant role in global visual impairment, underscoring the demand for innovative biomaterials with specific attributes such as adhesion, cohesion, and regenerative potential. In this study, we have developed a biocompatible
bioadhesive for corneal reconstruction. Derived from Collagen type I, naturally present in human corneal stromal tissue, the
bioadhesive was cross-linked with modified polyethylene glycol diacrylate (PEGDA-DOPA), rendering it curable through visible light exposure and exhibiting superior adhesion to biological tissues even in wet conditions. The physicochemical characteristics of the proposed
bioadhesive were customized by manipulating the concentration of its precursor polymers and adjusting the duration of photocrosslinking. To identify the optimal sample with maximum adhesion, mechanical strength, and biocompatibility, characterization tests were conducted. The optimal specimen, consisting of 30 % (w/v) PEGDA-DOPA and cured with visible light for 5 min, exhibited commendable adhesive strength of 783.6 kPa and shear strength of 53.7 kPa, surpassing that of commercialized eye adhesives.Additionally, biocompatibility test results indicated a notably high survival rate (>100 %) of keratocytes seeded on the hydrogel adhesive after 7 days of incubation. Consequently, this designed
bioadhesive, characterized by high adhesion strength, robust mechanical strength, and excellent biocompatibility, is anticipated to enhance the spontaneous repair process of damaged corneal stromal tissue.