■难以愈合的伤口,比如压力性溃疡和糖尿病性溃疡,是伤口敷料的主要挑战。这项研究的目的是开发一种基于聚合物和天然材料的具有独特生物学和治疗性能的生物活性敷料。
■敷料由含有聚乙烯醇(PVA)的活性层组成,蜂蜜,姜黄素和角蛋白,和具有较低亲水性的上层,其包含PVA以诱导柔性。通过傅里叶变换红外光谱表征敷料的物理化学性质,场发射扫描电子显微镜,膨胀行为和抗菌测量。使用实验性大鼠模型进行了伤口愈合研究,并将两种不同的生物活性敷料与商业伤口敷料进行了比较(Comfeel,Coloplast,丹麦)。为此目的进行组织病理学评价。
表征结果表明,产生了具有两个均匀但不同的层的光滑双层膜。该敷料还为伤口环境提供了足够的水分,而没有感染和由于干燥发生的粘附。我们的结果对革兰氏阴性(大肠杆菌)和革兰氏阳性(金黄色葡萄球菌)细菌表现出显着的杀菌活性,并改善了伤口愈合过程而没有任何疤痕。组织病理学发现表明,体内愈合率明显较高,表皮形成良好,肉芽组织形成和组织收缩,与商业伤口敷料相比。
■我们的结果表明,新型生物活性伤口敷料的物理和愈合效果可接受;然而,建议进行更多调查。
UNASSIGNED: Hard-to-heal wounds, such as pressure ulcers and diabetic ulcers, are a major challenge for wound dressings. The aim of this study was to develop a bioactive dressing based on polymers and natural materials with unique biological and therapeutic properties.
UNASSIGNED: The dressing was composed of an active layer containing polyvinyl alcohol (PVA), honey, curcumin and keratin, and an upper layer with lower hydrophilicity comprising PVA to induce flexibility. Physicochemical properties of the dressing were characterised by Fourier transform infrared spectroscopy, field emission scanning electron microscopy, swelling behaviour and antibacterial measurements. A wound healing study was performed using an experimental rat model and two different compositions of the bioactive dressing were compared with a commercial wound dressing (Comfeel, Coloplast, Denmark). Histopathological evaluation was conducted for this purpose.
UNASSIGNED: Characterisation results showed that a smooth bilayer film with two homogenous but distinct layers was produced. The dressing also provided adequate moisture to the wound environment without infection and adhesion due to dryness occurring. Our results exhibited significant bactericidal activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria and improved the wound healing process without any scarring. Histopathological findings demonstrated a significant higher healing rate in vivo together with well-formed epidermis, granulation tissue formation and tissue contraction, when compared with the commercial wound dressing.
UNASSIGNED: Our results demonstrated acceptable physical and healing effects for the novel bioactive wound dressing; however, more investigations are recommended.