beer

啤酒
  • 文章类型: Journal Article
    虽然健康的社会决定因素已经指导了公平工作与男性健康促进计划的剪裁,的作用,和潜力,这些干预措施中健康的商业决定因素很少得到解决,也知之甚少。虽然四种商业产品,烟草,酒精,超加工食品,和化石燃料,占全球死亡人数的三分之一以上,有必要认识到消费品行业可以对健康做出积极和消极的贡献。这篇文章开始了关于我们可以从中学到什么的急需的讨论,战略性地利用商业部门来播种,scale,并维持男性的健康促进计划。三个案例研究,在线体育博彩,啤酒和诺尼的崛起,和运动服,正在讨论。在线体育博彩和男性之间的联系解释了年轻人不成比例的参与和赌博成瘾,并建议立法终止赌博广告,并通过罚款和更高的税收来激励行业暴利。关于啤酒和诺尼的崛起,酿酒商基于其核心市场男性的消费模式和男性气概的变化,对无酒精啤酒进行了创新。Nonny提醒健康促进者了解其最终用户的价值观和行为,以增强程序的可接受性。详细介绍盔甲和露露柠檬,两个高度性别化但多样化的运动服装品牌,的复杂性,和潜力,强调了利用公共卫生和行业合作。一起来看,文章的研究结果表明,男性健康促进者应严格探索利用关键的商业实体和税收,以促进男性及其社区的健康。
    Although the social determinants of health have guided equity work with the tailoring of men\'s health promotion programs, the role of, and potential for, the commercial determinants of health in those interventions is rarely addressed and poorly understood. While four commercial products, tobacco, alcohol, ultra-processed food, and fossil fuels, account for more than a third of global deaths, there is a need to recognize that consumer goods industries can make both positive and negative contributions to health. This article begins much-needed discussions about what we might learn from, and strategically tap in the commercial sector to seed, scale, and sustain men\'s health promotion programs. Three case studies, online sports betting, beer and the rise of the nonny, and athleisurewear, are discussed. Connections between online sports betting and masculinities explain young men\'s disproportionate involvement and gambling addictions with recommendations to legislate an end to gambling advertisements and de-incentivize industry profiteering through penalties and higher taxes. Regarding beer and the rise of the nonny, brewers have innovated with non-alcoholic beer based on shifting consumption patterns and masculinities in their core market-men. The nonny reminds health promoters to know their end-user\'s values and behaviors to bolster program acceptability. Detailing Under Armour and Lululemon, two highly gendered but diversifying athleisurewear brands, the complexities of, and potential for, leveraging public health and industry collaborations are underscored. Taken together, the article findings suggest men\'s health promoters should rigorously explore tapping key commercial entities and tax revenues to advance the health of men and their communities.
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  • 文章类型: Journal Article
    啤酒的世界是由不同风格编织而成的丰富挂毯,每个人都有其独特的性格。Lager,以其脆度而闻名,在较低温度下发酵,而啤酒,在温暖的地方,拥有广泛的香气。比利时啤酒因其复杂性而令人眼花缭乱,从果味的Trappist啤酒到酸味的羊肉。德国小麦啤酒,像Hefeweizens,魅力与他们的泡腾和果味的底色。印度苍白的艾尔斯(IPA)展示了一个跳跃的爆发,而酸麦芽酒则以其浓郁而诱人。工艺,历史,和区域成分交织在这个酿造世界中,为爱好者提供一系列愉快的体验。对精酿啤酒香气的研究有限,分子指纹可能至关重要。迄今为止,没有研究集中在表征化合物的特征来区分啤酒的风格。顶空固相微萃取(HS-SPME)方法提供了一种快速无溶剂的挥发性化合物方法。本研究旨在通过使用HS-SPME/GC-MS技术结合多变量数据处理来表征各种啤酒的香气特征。总共收集了120个啤酒样品,并将其分为五类:比尔森(n=28);拉格(n=23);阿莱(n=32);酸(n=24);和比利时的艾尔斯(n=13)。在比尔森啤酒中,在含有啤酒花提取物和啤酒花的啤酒中发现了18种独特的化合物,和2用于含有啤酒花提取物(乙酸辛酯;和α-松油醇)的啤酒。当将其余的组相互比较时,比利时啤酒展示了5种独特的化合物,Lagers有一个(非肛门)。Sours和Ales没有独特的化合物,但与比利时组共享2种不同的化合物。我们得出的结论是,就各种与香气有关的化合物而言,比利时啤酒是最复杂的,并且可以从啤酒花提取物中区分使用纯啤酒花的啤酒。
    The world of beer is a rich tapestry woven with diverse styles, each with its unique character. Lager, known for its crispness, ferments at lower temperatures, while ale, at warmer ones, boasts a wide spectrum of aromas. Belgian beers dazzle with their complexity, from fruity Trappist ales to sour lambics. German wheat beers, like hefeweizens, charm with their effervescence and fruity undertones. India Pale Ales (IPAs) showcase a hoppy burst, while sour ales tantalize with their tanginess. Craftsmanship, history, and regional ingredients intertwine in this world of brewing, offering aficionados an array of delightful experiences. Research on craft beer aromas is limited, and molecular fingerprint could be crucial. To date, there have been no studies focused on characterizing compound profiles to differentiate beer styles. The Headspace Solid Phase Microextraction (HS-SPME) method provides a rapid and solvent-free approach to volatile compound. The present study aims to characterize the aroma profile of a wide range of beers by using HS-SPME/GC-MS technique combined with multivariate data processing. A total of 120 beer samples were collected and divided into five categories: Pilsen (n = 28); Lager (n = 23); Ale (n = 32); Sour (n = 24); and Belgian Ales (n = 13). Among the Pilsen beers, 18 unique compounds were found for beers with hop extract and hops, and 2 for beers with hop extract (Octyl acetate; and alpha-Terpineol). When comparing the remaining groups to each other, Belgian beers exhibited 5 unique compounds, and Lagers had one (nonanal). Sours and Ales did not have unique compounds but shared 2 distinct compounds with the Belgian group each. We concluded that Belgian beers are the most complex in terms of various aroma-related compounds, and that it is possible to distinguish beers that use pure hops from hop extract.
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  • 文章类型: Journal Article
    在捣碎过程之前去除外壳,也被称为Kubessa方法,是一种既定的酿造实践,通常与啤酒的光滑度和更好的风味稳定性呈正相关。关于Kubessa方法对啤酒影响的经验证据,然而,一直缺乏。同样,我们的研究对已建立的酿造属性的综合分析显示,传统的方法并不能完全捕获果壳分离在啤酒酿造中的影响。通过超高分辨率质谱(FT-ICR-MS)获得了Kubessa方法对啤酒老化化学影响的确凿证据,揭示了传统分析技术无法获得的复杂分子细节。解析了Kubessa啤酒中数千种分子的组成信息,并将其与全麦芽糖化进行了比较。应用于老化实验的机器学习算法确定了超过500种与老化相关的化合物被外壳分离抑制。与色谱联用的互补飞行时间质谱(ToF-MS)进一步证实,壳的糊化引入含硫脂质化合物。啤酒成分的这些显着差异为进一步研究啤酒生产过程中外壳分离(Kubessa过程)的老化保护作用提供了有价值的见解。正如在这项工作中实证证明的那样。
    The removal of husks before the mashing process, also known as the Kubessa method, is an established brewing practice often positively associated with smoothness and better flavor-stability of beer. Empirical evidence on the effect of the Kubessa method on beer, however, has been lacking. Similarly, our study\'s comprehensive analysis of established brewing attributes revealed that traditional methods do not fully capture the impact of husk separation in beer brewing. Conclusive evidence of the Kubessa method\'s impact on beer aging chemistry was obtained through ultrahigh resolution mass spectrometry (FT-ICR-MS), revealing intricate molecular details inaccessible to conventional analytical techniques. The compositional information on thousands of molecules in Kubessa beer was resolved and compared to whole malt mashing. Machine learning algorithms applied to aging experiments identified over 500 aging-related compounds inhibited by husk separation. Complementary Time of flight mass spectrometry (ToF-MS) coupled with chromatography further confirmed that the mashing of husks introduces sulfur-containing lipid compounds. These significant differences in the beer composition provide valuable insights for further investigation into the staling protective effect of husk-separation (Kubessa process) during beer production, as empirically demonstrated in this work.
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  • 文章类型: Journal Article
    已经开发了一种新的基于纳米孔测序的方法,用于检测和鉴定更广泛的微生物。该方法使用通用引物通过常见核糖体DNA区域的扩增和核苷酸测序来鉴定几乎所有细菌或酵母/真菌物种。其协议的简单性使得该方法适用于小型和大型啤酒厂。
    A new nanopore sequencing-based method has been developed for the detection and identification of a wider range of microorganisms. This method uses universal primers to identify virtually all the bacterial or yeast/fungal species via the amplification and nucleotide sequencing of common ribosomal DNA regions. The simplicity of its protocol makes the method suitable for both small and large breweries.
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  • 文章类型: Journal Article
    啤酒,作为一种古老而广泛消费的酒精饮料,拥有丰富的文化遗产和历史。近年来,水果啤酒作为一种独特的啤酒类型,通过将果汁加入传统啤酒成分中而获得了极大的关注。本研究采用顶空固相微萃取-气相色谱-质谱技术,冗余分析,和正交投影到潜在结构判别分析来分析感官评价,物理化学性质,有机酸,与不同比例的枇杷汁的枇杷啤酒和挥发性有机化合物(VOCs)。结果表明,添加适量的枇杷汁(40%)提高了啤酒的整体感官品质;随着枇杷汁比例的增加,苹果酸和酒石酸含量显著增加(p<0.05)。共鉴定出100种VOCs,其中23个关键VOCs(VIP>1,p<0.05)代表了枇杷啤酒中最重要的特征风味成分。本研究对枇杷的增值加工和经济发展具有重要意义。
    Beer, as an ancient and widely consumed alcoholic beverage, holds a rich cultural heritage and history. In recent years, fruit beer has gained significant attention as a distinct beer type produced by incorporating fruit juice into traditional beer ingredients. This study employed headspace solid-phase microextraction-gas chromatography-mass spectrometry techniques, redundancy analysis, and orthogonal projections to latent structures discriminant analysis to analyze the sensory evaluation, physicochemical properties, organic acids, and volatile organic compounds (VOCs) of loquat beer with different proportions of loquat juice. The results shown that the addition of an appropriate amount of loquat juice (40%) enhanced the overall sensory quality of the beer; as the proportion of loquat juice increased, the contents of malic acid and tartaric acid significantly increased (p < 0.05). A total of 100 VOCs were identified, among which 23 key VOCs (VIP > 1, p < 0.05) represented the most important characteristic flavor components in loquat beer based on their odor activity value (OAV). This study holds significant importance for the value-added processing and economic development of loquat.
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  • 文章类型: Journal Article
    啤酒的瓶子调理是额外的发酵步骤,其中将酵母和可发酵提取物添加到啤酒中用于碳酸化。在这个过程中,酵母必须克服环境压力,以确保在瓶中充分发酵。此外,酵母必须能够长时间存活,因为生存能力的下降将导致产品的改变。这里,我们使用六种不同的酿酒酵母菌株研究了瓶子调理对啤酒的影响,酿酒,蒸馏工业超过120天。啤酒和啤酒品系产生了具有淡啤酒风格啤酒典型特征的啤酒,而起泡酒和蒸馏酵母菌株产生的香气是不典型的,这是意料之中的。此外,我们观察到各种菌株在瓶子调理过程中具有不同的存活倾向。进行蛋白质组学分析以确定蛋白质丰度变化并揭示可能使特定酵母菌株在二次发酵期间存活更长时间的生物过程。我们的结果表明,与氧化还原酶活性和线粒体核糖体相关的蛋白质在酵母菌株中增加,具有优异的存活率,并且能够更有效地响应细胞应激,而与细胞壁调节相关的蛋白质在存活特性较差的菌株中增加。总的来说,我们证明了在这些条件下,酵母选择对瓶子调节的影响以及酵母生理学中涉及的生物过程。
    Bottle conditioning of beer is an additional fermentation step where yeast and fermentable extract are added to the beer for carbonation. During this process, yeast must overcome environmental stresses to ensure sufficient fermentation in the bottle. Additionally, the yeast must be able to survive for a prolonged time, as a decline in viability will lead to alterations in the product. Here, we investigated the effects of bottle conditioning on beer using six different yeast strains from the brewing, wine making, and distilling industries over 120 days. The ale and lager strains resulted in a beer possessing typical characteristics of a pale ale-style beer, whereas sparkling wine and distilling yeast strains resulted in aromas that were uncharacteristic, which was expected. In addition, we observed that the various strains had different propensities to survive during bottle conditioning. Proteomic analysis was performed to ascertain protein abundance changes and reveal biological processes that potentially enabled specific yeast strains to survive longer during secondary fermentation. Our results showed that proteins associated with oxidoreductase activity and mitochondrial ribosomes were increased in the yeast strain with superior survival and were able to respond to cellular stress more effectively, whereas proteins associated with cell wall modulation were increased in the strain with poor survival characteristics. Overall, we demonstrated the impact of yeast selection on bottle conditioning and the biological processes involved in yeast physiology under these conditions.
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  • 文章类型: Journal Article
    在酿造过程中,甲硫氨酸是形成(异味)风味的决定性氨基酸。甲硫氨酸的重要部分在麦芽中被氧化成甲硫氨酸亚砜(MetSO)。我们假设MetSO和MetSO2在酵母发酵过程中被代谢为挥发性化合物,并检查了酿酒酵母是否能够以游离和二肽结合的形式分解代谢l-MetSO和l-MetSO2。我们还研究了1-甲硫氨酸亚砜胺和S-甲基甲硫氨酸的稳定性。在测试化合物存在下的细胞活力为至少90%。游离和肽结合的测试物质均被酿酒酵母代谢。l-MetSO作为游离氨基酸降解最快,而l-MetSO2降解最快,结合在二肽中。我们观察到1-MetSO和1-甲硫氨酸亚砜胺的(R)和(S)非对映异构体的降解行为不同。此外,我们检测到甲硫氨酸是MetSO的唯一代谢产物。没有形成甲硫酚亚砜。MetSO2不转化为甲硫氨酸醇或甲硫氨酸砜,而是转化为相应的α-羟基酸。我们得出的结论是,MetSO还原为甲硫氨酸的过程比氨基转移快。酿造麦芽中MetSO或MetSO2的出现不会导致形成迄今为止未知的Ehrlich途径挥发性代谢物。
    In the brewing process, methionine is a decisive amino acid for (off-)flavor formation. A significant part of methionine is oxidized to methionine sulfoxide (MetSO) in malt. We hypothesized that MetSO and MetSO2 are metabolized to volatile compounds during yeast fermentation and examined whether the yeast Saccharomyces cerevisiae is able to catabolize l-MetSO and l-MetSO2 in free and dipeptide-bound forms. We also investigated the stability of l-methionine sulfoximine and S-methylmethionine. Cell viability in the presence of the test compounds was at least 90%. Both free and peptide-bound test substances were metabolized by Saccharomyces cerevisiae. l-MetSO was degraded most rapidly as the free amino acid, while l-MetSO2 was degraded most rapidly bound in dipeptides. We observed a different degradation behavior of the (R) and (S) diastereoisomers for l-MetSO and l-methionine sulfoximine. Furthermore, we detected methionol as the only metabolite of MetSO. Methionol sulfoxide was not formed. MetSO2 was not converted to methionol or methionol sulfone but to the respective α-hydroxy acid. We conclude that the reduction of MetSO to methionine proceeds faster than transamination. The occurrence of MetSO or MetSO2 in brewing malt will not lead to the formation of hitherto unknown volatile metabolites of the Ehrlich pathway.
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  • 文章类型: Journal Article
    本研究着眼于甘蔗产业的多元化利用,和甘蔗糖浆,作为甘蔗工业的副产品,是一种良好的发酵原料。将甘蔗糖浆带入啤酒有利于糖业的致富,它可以改善啤酒的味道,使其更加芳香。本研究确定了啤酒的最佳发酵工艺。通过分析原材料的碳源和氮源的消耗率,酵母的营养利用,以及风味物质差异的原因,采用SPME-HS-GC-MS技术对啤酒的风味成分和风味稳定性进行了测定。结果表明,以甘蔗糖浆为辅助原料酿造的啤酒符合啤酒的基本规格。向麦芽汁基质中添加甘蔗糖浆增加了酵母对氨基酸的利用率,LS(加入甘蔗糖浆的啤酒)增加了啤酒的九种风味化合物,构成了啤酒的基本风味,与普通的全大麦啤酒相比,带来了新的风味化合物。强制老化实验表明,LS产生的老化化合物比OWBL少。各种实验表明,用甘蔗糖浆代替部分麦芽汁发酵啤酒是可行的。
    This study focuses on the diversified utilization of the sugarcane industry, and sugarcane syrup, as a by-product of the sugarcane industry, is a good raw material for fermentation. Bringing sugarcane syrup into beer is conducive to the enrichment of the sugar industry, and it can improve the flavor of beer and make it more aromatic. This study determined the optimal fermentation process for beer. By analyzing the consumption rate of the carbon and nitrogen sources of raw materials, the nutrient utilization of yeast, and the causes of differences in flavor substances, the flavor composition and flavor stability of beer were determined by SPME-HS-GC-MS technology. The results showed that beer brewed with sugarcane syrup as an auxiliary raw material met the basic specifications of beer. The addition of sugarcane syrup to the wort base increased the utilization of amino acids by the yeast, and LS (lager with added cane syrup) increased the nine flavor compounds of the beer, which constituted the basic flavor of the beer, bringing new flavor compounds compared with the normal all-barley beer. Forced aging experiments showed that LS produced fewer aging compounds than OWBL. Various experiments have shown that it is feasible to ferment beer with sugarcane syrup instead of partial wort.
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  • 文章类型: Journal Article
    酒糟(BSG)是酿酒行业的主要副产品,占啤酒生产过程中产生的总废物的85%。这种木质纤维素材料传统上用作家畜饲料并以低价出售。然而,BSG可用作生产生物活性分子和化学品前体的低成本原料,提升这种副产品的价值。在这种情况下,BSG是用于提取抗氧化剂如阿魏酸(FA)和对香豆酸(p-Cu)的有前途的原料。评估了三种水解处理从BSG中提取FA和p-Cu的有效性,即酶(基于阿魏酸酯酶和内切-1,4-β-木聚糖酶之间的协同合作),碱性和热液。水热处理产生了最高的提取率(FA和p-Cu的7.2g/kgBSG和1.4g/kgBSG,分别)在较短的提取时间(一小时)内。另一方面,在25°C孵育4小时内,酶水解提取了4.3g/kgBSG的FA,p-Cu的产量可忽略不计。在120°C下碱处理超过5小时,FA的产率为5.5g/kgBSG,p-Cu的产率为0.6g/kgBSG。质量和能量平衡表明,操作成本对提取过程中使用的BSG浓度的高度依赖性。费用为34.5欧元,该化学品每公斤FA为6607欧元和205.5欧元,100kgBSG/m3的酶和水热萃取方法。
    Brewers\' spent grain (BSG) is the main byproduct from the brewing industry, which accounts for 85 % of the total waste generated during beer production. This lignocellulosic material is traditionally used as livestock feed and sold at a low price. However, BSG can be used as a low-cost feedstock for the production of bioactive molecules and chemicals precursors, upgrading the value of this byproduct. In this context, BSG is a promising feedstock for the extraction of antioxidants like ferulic acid (FA) and p-coumaric acid (p-Cu). The effectiveness of three hydrolysis treatments were evaluated for the extraction of FA and p-Cu from BSG, namely enzymatic (based on the synergistic cooperation between a feruloyl esterase and an endo-1,4-β-xylanase), alkaline and hydrothermal. The hydrothermal treatment produced the highest extraction yields (7.2 g/kgBSG and 1.4 g/kgBSG for FA and p-Cu, respectively) in a short extraction time (an hour). On the other hand, enzymatic hydrolysis extracted 4.3 g/kgBSG for FA and negligible yields for p-Cu in 4 h of incubation at 25 °C. Yields of 5.5 g/kgBSG for FA and 0.6 g/kgBSG for p-Cu were obtained in more than 5 h of alkaline treatment at 120 °C. The mass and energy balances revealed the high dependence of the operating costs on the concentration of BSG used during the extraction process, with costs of 34.5 €, 6607 € and 205.5 € per kg of FA for the chemical, enzymatic and hydrothermal extraction methods at 100 kg BSG/m3.
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  • 文章类型: Journal Article
    减轻农业和工业副产品对人口和环境的不利影响至关重要。不断探索升级和重新设计这些副产品的方法至关重要。布鲁尔的废谷物(BSG),啤酒酿造过程的主要副产品,约占这些副产品的85%。其高水分含量和丰富的营养特征使BSG成为微生物利用的有希望的候选者。因此,提高高产量,通过微生物发酵的低成本BSG增加了显著的价值。本文全面概述了通过微生物处理实现BSG的两种增值途径,定制所需的最终产品:利用发酵的BSG作为人类或动物饮食中的营养补充剂,或以BSG为底物培养食用菌。该综述还探讨了BSG的微生物发酵以产生有价值的代谢产物,为其高价值利用奠定理论基础。
    Mitigating the adverse impacts of agricultural and industrial by-products on human populations and the environment is essential. It is crucial to continually explore methods to upgrade and reengineer these by-products. Brewer\'s Spent Grain (BSG), the primary by-product of the beer brewing process, constitutes approximately 85% of these by-products. Its high moisture content and rich nutritional profile make BSG a promising candidate for microbial utilization. Consequently, valorizing high-yield, low-cost BSG through microbial fermentation adds significant value. This paper provides a comprehensive overview of two valorization pathways for BSG via microbial processing, tailored to the desired end products: utilizing fermented BSG as a nutritional supplement in human or animal diets, or cultivating edible fungi using BSG as a substrate. The review also explores the microbial fermentation of BSG to produce valuable metabolites, laying a theoretical foundation for its high-value utilization.
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