食源性致病菌可导致食品腐败变质,导致食品安全问题。近年来,食品包装受到了很多关注。传统的包装膜是不可生物降解的,并且在环境中长时间存在。在这项研究中,以蒸馏水为溶剂,通过绿色提取工艺从五味子中提取天然抗菌物质,并比较不同处理方法对提取物抑菌活性的影响。同时,以聚乙烯醇(PVA)为基材,制备了四种类型的五味子抗菌膜。整个提取和膜制备过程不涉及有机溶剂,使过程绿色环保。材料表征包括倒置生物显微镜,扫描电子显微镜(SEM),原子力显微镜(AFM),傅里叶变换红外光谱(FTIR),抗拉强度试验,孔径测量,吸水率试验,等。其中,在MⅡ和MⅣ表面用肉眼观察不到萃取物颗粒。MⅡ具有均匀透明,几乎无色的形态,是最有张力的。MⅣ表面平整光滑,微观结构致密均匀。同时,对四种膜进行了12小时的常见致病菌检测,和OD600趋势揭示了膜对金黄色葡萄球菌的优异的抗菌活性,MRSA,大肠杆菌,和L.单核细胞增生。膜也可以重复使用至少一次。本研究为天然植物性抗菌膜的制备提供了新的思路。
Foodborne pathogens can cause food spoilage and lead to food safety issues. In recent years, food packaging has received a lot of attention. Traditional packaging membranes are non-biodegradable and remain in the environment for a long time. In this study, natural antimicrobial substances were extracted from Schisandra chinensis by a green extraction process using distilled water as the solvent, and the effects of different treatment on the antimicrobial activity of the extract were compared. At the same time, four types of Schisandra chinensis antimicrobial membranes were prepared using polyvinyl alcohol (PVA) as the substrate. The whole extraction and membrane preparation process did not involve organic solvents, making the process green and environment friendly. Material characterization included inverted biological microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), tensile strength test, pore size measurement, water uptake test, etc. Among them, no extract particles were observed with the naked eye on the surfaces of MⅡ and MⅣ. MⅡ has a uniformly transparent, nearly colorless morphology and is the most tensile. MⅣ surface is flat and smooth, the microstructure is dense and uniform. At the same time, the four types of membranes were tested against common pathogenic bacteria for 12 h, and the OD600 trend revealed the excellent antimicrobial activity of the membranes against S. aureus, MRSA, E. coli, and L. monocytogenes. The membranes could also be reused at least once. This study provides a new idea for preparing natural plant-based antimicrobial membranes.