PDF(Pubmed)
Abstract：
Photodynamic therapy (PDT) has been based on using photosensitizers (PS) and applying light of a specific wavelength. When this technique is used for treating infections, it is known as antimicrobial photodynamic therapy (aPDT). Currently, the use of lighting sources for in vitro studies using aPDT is generally applied in multiwell cell culture plates; however, depending on the lighting arrangement, there are usually errors in the application of the technique because the light from a well can affect the neighboring wells or it may be that not all the wells are used in the same experiment. In addition, one must be awarded high irradiance values, which can cause unwanted photothermal problems in the studies. Thus, this manuscript presents an in vitro antimicrobial photodynamic therapy for a Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA) inhibition study using an arrangement of thermally isolated and independently illuminated green light source systems for eight tubes in vitro aPDT, determining the effect of the following factors: (i) irradiance level, (ii) exposure time, and (iii) Rose Bengal (RB) concentration (used as a PS), registering the Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA) inhibition rates. The results show that in the dark, RB had a poor antimicrobial rate for P. aeruginosa, finding the maximum inhibition (2.7%) at 30 min with an RB concentration of 3 µg/mL. However, by applying light in a correct dosage (time × irradiance) and the adequate RB concentration, the inhibition rate increased by over 37%. In the case of MRSA, there was no significant inhibition with RB in complete darkness and, in contrast, the rate was 100% for those experiments that were irradiated.
摘要：
光动力疗法(PDT)基于使用光敏剂(PS)和施加特定波长的光。当这种技术用于治疗感染时，它被称为抗微生物光动力疗法(aPDT)。目前,使用aPDT进行体外研究的光源通常应用于多孔细胞培养板；然而，根据照明布置，该技术的应用通常存在错误，因为来自井的光可能会影响相邻的井，或者可能不是所有的井都在同一实验中使用。此外,必须获得高辐照度值，这可能会导致研究中不必要的光热问题。因此，本手稿提出了一种铜绿假单胞菌的体外抗菌光动力疗法（P.铜绿假）和耐甲氧西林金黄色葡萄球菌（MRSA）抑制研究，使用热隔离和独立照明的绿色光源系统，用于八个体外aPDT管，确定以下因素的影响：(I)辐照度水平，(ii)暴露时间，和（iii）玫瑰红（RB）浓度（用作PS），注册铜绿假单胞菌(P.铜绿假单胞菌）和耐甲氧西林金黄色葡萄球菌（MRSA）的抑制率。结果表明，在黑暗中，RB对铜绿假单胞菌的抗菌率差，在RB浓度为3µg/mL的30分钟时发现最大抑制作用（2.7％）。然而,通过以正确的剂量（时间×辐照度）和足够的RB浓度施加光，抑制率提高了37%以上。在MRSA的情况下，在完全黑暗的情况下，RB没有明显的抑制作用，相比之下,对于那些被辐照的实验，比率是100%。
