UNASSIGNED:本研究旨在了解碳青霉烯酶基因的分布特征,并评估氨曲南/阿维巴坦(ATM/AVI)和头孢他啶/阿维巴坦(CAZ/AVI)对碳青霉烯类耐药肠杆菌(CRE)的抗菌活性。中国西南地区。
UNASSIGNED:CRE分离株及其临床信息是在2016年1月1日至2017年12月31日期间从覆盖重庆所有五个地区的22家医院收集的。PCR用于筛选常见的碳青霉烯酶基因。并通过肉汤微量稀释法测定最小抑制浓度(MIC)。
未经鉴定:总共312个未重复的CRE分离株(例如,206肺炎克雷伯菌,43大肠杆菌,在两年的研究期间收集了42个阴沟肠杆菌)。在这些CRE分离物中,92.3%携带碳青霉烯酶基因,大多数分离株携带单个blaKPC-2(47.1%)或单个blaNDM/IPM(36.2%),而8.9%的分离株携带两个或三个碳青霉烯酶基因。值得注意的是,95.6%(197/206)肺炎克雷伯菌,86.0%(37/43)大肠杆菌和88.1%(37/42)阴沟肠杆菌具有碳青霉烯酶基因。此外,BLAKPC-2在肺炎克雷伯菌中普遍存在(70.4%),而BlaNDM在大肠杆菌(83.7%)和阴沟肠杆菌(78.6%)中占主导地位。此外,在儿童CRE分离株中仅检测到金属β-内酰胺酶(MBL)基因。总的来说,0.0%,48.1%,59.0%,61.5%和63.1%的CRE分离株对ATM/AVI耐药,CAZ/AVI,呋喃妥因,阿米卡星和甲氧苄啶/磺胺甲恶唑,分别。MIC为1μg/mL的ATM/AVI可杀死312株中的99.7%,而CAZ/AVI对blaKPC-2载体显示出良好的抗菌活性(98.0%敏感),MIC50/90值为1/4μg/mL。
UNASSIGNED:在这项涵盖重庆所有地理位置的多中心研究中,首次从CRE的种类和来源方面全面阐述了碳青霉烯酶基因在重庆的分布特征。ATM/AVI对所有CRE分离株显示出优越的活性,无论其基因型如何,而CAZ/AVI对几乎所有KPC生产者都很活跃。
UNASSIGNED: This study aimed to understand the distribution characteristics of carbapenemase genes and assess the antimicrobial activities of aztreonam/avibactam (ATM/AVI) and ceftazidime/avibactam (CAZ/AVI) against carbapenem-resistant Enterobacterales (CRE) isolates in Chongqing, Southwest China.
UNASSIGNED: CRE isolates and their clinical information were collected from 22 hospitals covering all the five regions across Chongqing between January 1, 2016 and December 31, 2017. PCR was used to screen for common carbapenemase genes. And minimum inhibitory concentrations (MICs) were determined by broth microdilution method.
UNASSIGNED: A total of 312 unduplicated CRE isolates (eg, 206 Klebsiella pneumoniae, 43 Escherichia coli, and 42 Enterobacter cloacae) were collected during the two-year study period. Among these CRE isolates, 92.3% carried carbapenemase genes, with a majority of isolates carrying single bla KPC-2 (47.1%) or single bla NDM/IPM (36.2%) and 8.9% of isolates carrying two or three carbapenemase genes. Notably, 95.6% (197/206) K. pneumoniae, 86.0% (37/43) E. coli and 88.1% (37/42) E. cloacae harbored carbapenemase genes. In addition, bla KPC-2 was prevalent in K. pneumoniae (70.4%), while bla NDM was predominant in E. coli (83.7%) and E. cloacae (78.6%). Besides, only metallo-β-lactamase (MBL) genes were detected in the CRE isolates from children. Overall, 0.0%, 48.1%, 59.0%, 61.5% and 63.1% of the CRE isolates were resistant to ATM/AVI, CAZ/AVI, nitrofurantoin, amikacin and trimethoprim/sulfamethoxazole, respectively. 99.7% of the total 312 isolates could be killed by ATM/AVI with the MIC 1 μg/mL, whereas CAZ/AVI showed good antibacterial activity (98.0% susceptible) against the bla KPC-2-carriers with the MIC50/90 values of 1/4 μg/mL.
UNASSIGNED: The distribution features of carbapenemase genes in Chongqing were comprehensively illustrated in terms of species and sources of CRE for the first time in this multi-center study that covered all the geographical locations across Chongqing. ATM/AVI showed superior activity against all CRE isolates regardless of their genotype, whereas CAZ/AVI was active against almost all KPC-producers.